Comparative Identification of LjR2R3-MYB Family Based on Two Generations of Lotus corniculatus Genomes
[Objective]The structure and function of LjR2R3-MYB transcription factor family were explored to provide a reference for the study of the differences between second-generation sequencing and third-generation sequencing,the exploration and identification of transcription factor in Lotus corniculatus,and the identification of the regulatory functions of LjR2R3-MYBs.[Method]The LjR2R3-MYB transcription factors of two versions of the whole genome data of L.corniculatus from second-generation sequencing(SGS)and third-generation sequencing(TGS)were identified and analyzed,and the composition of their members,evolutionary features,gene structure,protein properties,spatial structure,chromosomal localization,cis-acting elements were studied.[Result]In the SGS and TGS versions,the LjR2R3-MYB family had 96 and 135 members,respectively,and the gene sequences of TGS had 3 more conserved motifs than that of SGS;The family members all belonged to 14 subfamilies,and TGS obtained 39 more unique sequences than that of SGS;There were 86 genes in SGS that contained UTR,and 78 genes in TGS,and the motif types of LjR2R3-MYB member were different;All LjR2R3-MYB genes encoded proteins were hydrophilic,without signal peptide,located in the nucleus,and had basically the same secondary structural composition;25 R2R3-MYB genes were distributed on chromosome 0 in SGS,and TGS contained 6 chromosomes and no chromosome 0;The LjR2R3-MYB gene promoter contained cis-acting elements such as adversity stress,defense,hormones,and growth and developmental responses.[Conclusion]Compared with SGS,the amount of gene information obtained from TGS data was more complete and abundant,which confirmed that the assembly quality of TGS in L.corniculatus was higher than that of SGS.
NETL
NSTL
万方数据
