Spatial Distribution Characteristics of Ammonia-oxidizing Archaea and Ammonia-oxidizing Bacteria in Caohai Sediments Based on Fluorescence Quantitative PCR
[Objective]The abundance,community structure and distribution rules of nitrogen cycle microorganisms in Caohai surfacial sediment of Guizhou were explored,which provided the theoretical references for studying the nitrogen cycle mechanism of lake sediments and remedying lake ecology.[Method]The abundance of ammonia-oxidizing archaea(AOA)and ammonia-oxidizing bacteria(AOB)in Caohai surfacial sediments in different seasons was determined by real-time fluorescence quantitative PCR technology.Meanwhile,the physicochemical factors in the sediments,pore water and overlying water were determined,and the effects of various environmental factors on the distribution of AOA amoA and AOB amoA were analyzed.[Result]The abundance of AOA amoA gene and AOB amoA gene was 7.81×103 copies/g-4.11×106 copies/g and 3.50×102 copies/g-8.11×104 copies/g.The amoA copy number varied significantly in each sampling region,showing spatial distribution characteristics.The abundance of AOA amoA and AOB amoA genes was low in the lakeshore zone.The AOA/AOB in Caohai sediments ranged from 2.3 to 181.4.In addition,the overall organic matter content,total phosphorus and total nitrogen of Caohai sediments was 65.57-436.30 g/kg,3.870-5.640 g/kg and 0.585-1.252 g/kg,respectively.The concentration of ammonia nitrogen and nitrate nitrogen in pore water were 0.283-2.082 mg/L and 0.007-0.060 mg/L,respectively.[Conclusion]AOA plays an important role in Caohai ammoxidation process.The abundance of AOA amoA gene is affected by organic matter and total nitrogen,while that of AOB amoA gene is affected by dissolved oxygen and total phosphorus.
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