Analysis on Rhizosphere Microbial Diversity and Community Structure of Different Flue-cured Tobacco Varieties
[Objective]The rhizosphere bacterial community and fungal community structure of different flue-cured tobacco varieties were explored,and the relationship between flue-cured tobacco plants and rhizosphere microorganisms was studied,which provided theoretical basis for improving rhizosphere microecological environment of tobacco plants,promoting disease and pest control during tobacco production and improving tobacco quality.[Method]Using Zhongyan 100,NC89 and Yunyan 87 as experimental materials,Illumina high-throughput technology combined with the physicochemical properties of rhizosphere soil were used to analyze the differences of rhizosphere soil microbial community composition and diversity among different varieties of flue-cured tobacco plants.[Result]In the rhizosphere microbial community of tobacco,the four dominant phyla of rhizosphere soil bacteria were Actinobacteriota,Proteobacteria,Acidobacteriota and Chloroflexi.The two dominant phyla of rhizosphere soil fungi were Ascomycota and Mortierellomycota.The alpha diversity of cultivar NC89 was significantly higher than Zhongyan 100 and Yunyan 87,showing that NC89 bacteria diversity was superior to other two varieties,and the beneficial bacteria Mortierella,Neocosmospora,unclassified_k_Fungi content was higher than the other two varieties.[Conclusion]There are significant differences in the composition and diversity of bacterial and fungal communities in the rhizosphere soil of different flue-cured tobacco varieties.The diversity of bacterial and fungal communities in the rhizosphere soil of NC89 is better than that of Zhongyan 100 and Yunyan 87.
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