Establishment and Application of a Rapid Analysis Method for Oil Composition of Idesia polycarpa Maxim.var.vestita
[Objective]The qualitative and quantitative analysis methods for efficient and rapidly detecting triacylglycerols(TG)in Idesia polycarpa Maxim.var.vestita Diels oil were explored,which provided detection method and reference for identifying the quality and developing and utilizing of I.polycarpa oil comprehensively.[Method]The composition and content of TG in I.polycarpa oil from different regions were detected by three methods of UPLC-PDA,UPLC-MS and UPLC-ELSD.The sensitivity and selectivity of three methods were compared to determine the best determination method.[Result]UPLC-MS could quantitatively and qualitatively analyze 14 TG composition such as 1-heptadecanoic acid-2-arachidonic acid-3-linoleic acid,1,2-oleic acid-3-linoleic acid,1,-linoleic acid-2-oleic acid-3-linolenic acid from I.polycarpa oil,and the detection range and sensitivity were superior to UPLC-PDA and UPLC-ELSD.The application results of UPLC-MS showed that TG11 and TG14 were the main factors affecting the composition and content of TG in seed,pulp and fruit oil of I.polycarpa from 13 different producing areas.[Conclusion]UPLC-MS is the most suitable method for identifying TG in I.polycarpa oil,which can rapidly and accurately identify the composition,analyze and control the quality of I.polycarpa oil.
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