首页|芸苔素内酯浓度对干旱胁迫大白菜幼苗生长及生理指标的影响

芸苔素内酯浓度对干旱胁迫大白菜幼苗生长及生理指标的影响

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[目的]探明不同浓度芸苔素内酯对干旱胁迫大白菜幼苗抗旱性的调控作用,为芸苔素内酯在大白菜生产中的应用提供参考.[方法]以大白菜胜丽 118为试验材料,苗期喷施不同浓度(0.05 mg/L、0.1 mg/L、0.5 mg/L、1 mg/L)28-表高芸苔素内酯后,选用 10%浓度的聚二乙醇-6000溶液模拟干旱胁迫,测定大白菜生长和生理生化指标,分析不同浓度芸苔素内酯处理大白菜幼苗生理活性差异.[结果]随芸苔素内酯浓度升高,大白菜幼苗长势呈先良好后衰败变化;从大白菜幼苗表型看,芸苔素内酯浓度 0.05 mg/L、0.1 mg/L处理的耐旱效果最明显.干旱胁迫下叶片POD和CAT活性随芸苔素内酯浓度升高呈先增强后减弱趋势,MDA含量随浓度升高呈先降后升趋势.芸苔素内酯浓度过高大白菜幼苗叶绿素相对含量变低.[结论]喷施适宜浓度的芸苔素内酯可提高干旱胁迫下大白菜幼苗的抗氧化酶活性,有效缓解干旱胁迫造成的损害,适宜的芸苔素内酯浓度为0.05~0.1 mg/L.
Effects of Brassinolide Concentrations on Growth and Physiological Indexes of Chinese Cabbage Seedlings Under Drought Stress
[Objective]The regulation effects of brassinolide with different concentrations on drought resistance of Chinese cabbage seedlings under drought stress were studied,so as to provide reference for applying brassinolide during production of Chinese cabbage.[Method]The Chinese cabbage'Shengli 118'was selected as the test material,and polyethylene glycol-6000 solution with 10%concentration was selected to simulate drought stress.After spraying different concentrations(0.05 mg/L,0.1 mg/L,0.5 mg/L and 1 mg/L)of 28-homobrassinolide,the growth and physiochemical indexes were determined to analyze the differences in physiological activity of Chinese cabbage seedlings treated with different concentrations of brassinolide.[Result]With the increase of brassinolide concentration,the growth state of cabbage seedlings showed a trend of good at first and then decayed.Form the aspect of phenotype of Chinese cabbage,their drought tolerance was the most obvious when treated with 0.05 mg/L and 0.1 mg/L brassinolide.The POD and CAT activities of leaves under drought stress showed a tendency to enhance and then weaken with brassinolide concentration increasing,while the MDA content decreased and then increased.The high concentration of brassinolide resulted in lower relative chlorophyll content of Chinese cabbage seedlings.[Conclusion]Spraying appropriate concentrations of brassinolide can improve the antioxidant enzyme activities of cabbage seedlings under drought stress,effectively alleviate the damage caused by drought stress,and spraying brassinolide at 0.05 mg/L and 0.1 mg/L concentrations are the best.

Chinese cabbageseedlingsbrassinolidedrought stressenzyme activity

张新梅、赵文麟、和芬、许彬、许俊强、张国平

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云南农业大学 云南省滇台特色农业产业化工程研究中心,云南 昆明 650201

云南农业大学 园林园艺学院/云南省蔬菜生物学重点实验室,云南 昆明 650201

大白菜 幼苗 芸苔素内酯 干旱胁迫 酶活性

云南省农业联合专项云南省基础研究计划云南省重大科技计划云南省科技人才和平台计划云南省教育厅科研项目

202101BD070001-115202201AT070502202102AE090005202205AF1500172023Y0987

2024

贵州农业科学
贵州省农业科学院

贵州农业科学

CSTPCD
影响因子:0.642
ISSN:1001-3601
年,卷(期):2024.52(5)
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