后处理加工工艺对饲用凝结芽孢杆菌活菌收率的影响
Effects of the Post-processing Technique on Recovery Rate of Viable Bacteria of Bacillus coagulans for Feeding
曾发姣 1刘毅 2周小玲 1王升平 3舒燕 1龚平 1曾奥 1周映华 3高书锋3
作者信息
- 1. 湖南省微生物研究院,湖南 长沙 410009;饲用微生态制剂湖南省工程实验室,湖南 长沙 410009
- 2. 长沙市农业技术推广中心,湖南 长沙 410000
- 3. 湖南省微生物研究院,湖南 长沙 410009;饲用微生态制剂湖南省工程实验室,湖南 长沙 410009;湖南省农用微生物工程技术研究中心,湖南 长沙 410009
- 折叠
摘要
[目的]探明提高饲用凝结芽孢杆菌发酵后处理加工贮存过程中的活菌收率,为降低规模化生产成本提供科学依据.[方法]以饲用凝结芽孢杆菌(Bacillus coagulans)W-02菌株为研究对象,分别检测凝结芽孢杆菌发酵后处理加工贮存过程中离心浓缩工艺、干燥方式、粉碎时间和低温保存时间前后活菌数,计算各环节活菌收率,最终计算产品活菌总收率.[结果]经 4 000 r/min、15 min离心浓缩工艺,凝结芽孢杆菌菌泥平均活菌收率为 91.57%;经-58℃、100 mT真空冷冻干燥工艺,干燥产品活菌收率为 92.98%;经转速 24 000 r/min、粉碎 15 s,粉碎产品活菌收率为 83.37%;经 4℃低温保存 365 d,凝结芽孢杆菌活菌收率为 71.21%.[结论]饲用凝结芽孢杆菌发酵液经离心浓缩、吸附干燥、高速粉碎最优条件处理后,低温保存 365 d时活菌总收率(各环节活菌收率之积)为50.55%.
Abstract
[Objective]It aimed to study the development of recovery rate of viable bacteria of Bacillus coagulans for feeding during processing and storage after fermentation,which provided the scientific basis for reducing the cost of large-scale production.[Method]Taking the B.coagulans for feeding,W-02,as the study object,the centrifugal concentration process,drying method,smashing time and the viable bacterial before and after cryopreservation time during processing and storage after fermentation,and the total recovery rates of viable bacteria were counted.[Result]The average recovery rate of viable bacteria of B.coagulans was 91.57%after centrifugal concentration by 4 000 r/min for 15 min.The recovery rate of viable bacteria in dry B.coagulans was 92.98%by vacuum freeze-drying at-58℃and 100 mT.The recovery rate of viable bacteria in crushed B.coagulans was 83.37%through the smashing process at 24 000 r/min and 15 seconds.The recovery rate of viable bacteria of B.coagulans was 71.21%as stored at 4℃for 365 d.[Conclusion]After centrifugal concentration,sorption drying,high-speed crushing,the total recovery rate of viable bacteria of B.coagulans for feeding(the product of viable bacteria at each stage)is 50.55%as stored for 365 d with low temperature.
关键词
凝结芽孢杆菌/活菌收率/离心浓缩/干燥方式/粉碎时间/后处理加工/低温保存Key words
Bacillus coagulans/recovery rate of viable bacteria/centrifugal concentration/drying method/smash-ing time/post-processing techinique/low-temperature storage引用本文复制引用
基金项目
湖南省重点研发计划(2022NK2041)
长沙市自然科学基金(kq2208133)
出版年
2024
国家科技期刊平台
NSTL
万方数据