Study on Chemical Composition and Antioxidation of Cucumis metuliferus and Its Nematicidal Activity
[Objective]The chemical components,antioxidation and nematicidal activities of Cucumis metuliferus were explored to lay a theoretical foundation for the preparation of plant nematicides.[Method]The compounds were isolated and purified by silica gel,reversed-phase medium and low pressure,gel Sephadex LH-20 column chromatography and preparative high performance liquid chromatography,and identified with the physicochemical properties,various spectra and data reported in the literature,and then the nematicidal and antioxidant activities of the isolated compounds were analyzed.[Result]A total of four compounds were isolated from C.metuliferus,which were identified as isovitexin-2''-O-glucoside(compound 1),2E,4E-tridecadienedioic acid(compound 2),9E,11E-(13 oxo)tridecadienoic acid(compound 3)and 9E,11E-(13 hydroxy)tridecadienoic acid(compound 4).Compound 1 had a certain degree of antioxidant activity.When the mass concentration was 1 mg/mL,the scavenging rate of compound 1 to DPPH reached 49.40%.Compounds 1,3,and 4 exhibited a certain nematicidal activity.Compound 1 exhibited strong nematicidal activity at 2.0 mg/ml,with a mortality rate of 90.5%against nematodes.Compounds 3 and 4 had a mortality rate of 100%against nematodes at 0.25 mg/ml.[Conclusion]It is the first time that four compounds of isovitexin-2''-O-glucoside,2E,4E-tridecadienedioic acid,9E,11E-(13 oxo)tridecadienoic acid and 9E,11E-(13 hydroxy)tridecadienoic acid are isolated from C.metuliferus,among which 2E,4E-tridecadienedioic acid and 9E,11E-(13 oxo)tridecadienoic acid are new natural products.Three compounds of isovitexin 2''-O-glucoside,9E,11E-(13 oxo)tridecadienoic acid and 9E,11E-(13 hydroxy)tridecadienoic acid have nematicidal activity,while isovitexin-2''-O-glucoside has antioxidant activity.
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