Synergistic Effects of Sterilization and Seedling Age on Survival Rate and Growth Vigor of Corydalis saxicola Tissue Cultured Seedlings
[Objective]The synergistic effects of sterilization and seedling age on survival rate and growth of Corydalis saxicola seedlings were explored during tissue cultured process,which provided the theoretical basis for the efficient production of C.saxicola.[Method]The effects of sterilization and different number of true leaves(2,3,4,5,6)on the survival rate and growth vigor of C.saxicola seedlings were studied by taking C.saxicola seedlings as test materials.[Result]In all seedling age groups,the survival rate of tissue cultured seedlings under sterilization treatment increased by 7.10-42.75 percentage points with an average increase of 26.07 percentage points compared with unsterilization treatment.The growth indicators of transplanting seedlings at the same seedling age were not significantly affected by sterilization treatment.The number of true leaves was significantly and positively correlated with the survival rate of tissue cultured seedlings,and sterilization treatment and a number of true leaves between 3 and 6 could control the death rate of seedling to within 10%.In the sterilization treatment groups,the number of true leaves was also significantly and positively correlation with the root weight(0.63),root thickness(0.73),relative content of chlorophyll(0.55)and above-ground weight(0.69)of transplanting seedlings,and it was suitable for growth of true leaves between 3 and 6.Through analysis of the relative growth index(RGI)of the whole plant,the true leaf number was significant correlation with the whole plant RGI of C.saxicola transplanting seedlings,and the suitable number of true leaves was further determined to be 4.[Conclusion]The survival rate and growth of 4 true leaves of C.saxicolatissue cultured seedlings under sterilization treatment are the best.
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