Content Characteristics of Flavonoids in Spatholobus suberectus Planted Under Forest at Early Growth Stage
[Objective]The relationship between the growth performance and the content of the main medicinal components in the artificial cultivation of Spatholobus suberectus planted under the forest were studied,which provided a theoretical reference for planting management and harvesting S.suberectus planted under the forest.[Method]The contents of five flavonoid compounds(protocatechuic acid,catechin,epicatechin,liquiritigenin and formononetin)in 3-year-old S.suberectus with different diameter,branching classes and rattan preservation methods were detected by high performance liquid chromatography(HPLC)method.Analysis of variance(ANOVA),principal component analysis(PCA)and curvilinear regression analysis were used to reveal the difference and variation trend of the five flavonoids contents under different treatments.[Result]There were significant differences in four flavonoids among diameter classes of rattan of S.suberectus except epicatechin.The contents of protocatechuic acid,catechin and formononetin increased linearly as the diameter class of rattan increased,while the liquiritigenin non-linearly decreased with the diameter of rattan;The contents of protocatechuic acid,liquiritigenin and formononetin significantly differed among different branch grades of S.suberectus.The protocatechuic acid,catechin,formononetin and epicatechin decreased with the increase of branch grades,while the content of liquiritigenin showed a reverse tendency.The contents of catechin and epicatechin in air-dried samples of S.suberectus were significantly higher than those in refrigerated fresh samples.[Conclusion]The protocatechin acid,catechin and formononetin mainly exist in xylem.On the contrary,the liquiritigenin primarily locates at non-xylem area.The air-drying preservation of S.suberectusis beneficial to the accumulation of catechin and epicatechin.
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