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西藏不同地区大花黄牡丹生境土壤理化性质及酶活性研究

Study on Soil Physicochemical Properties and Enzyme Activity of Paeonia ludlowii Habitat in Different Areas of Tibet

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[目的]探明大花黄牡丹生境土壤理化性质和酶活性分布规律,为高原牡丹产业发展提供参考.[方法]以拉萨、山南和林芝 3个不同地区大花黄牡丹生境土壤为试材,通过测定其土壤含水量、pH、电导率、有机质、全氮、全磷、全钾、碱解氮、有效磷、速效钾含量及土壤β-N-乙酰氨基葡萄糖苷酶(NAG)、β-葡萄糖苷酶(BG)、亮氨酸氨基肽酶(LAP)、过氧化物酶(POD)和多酚氧化酶活性(PPO),分析大花黄牡丹对土壤的适应性.[结果]拉萨、山南和林芝 3个地区大花黄牡丹生境土壤pH为 6.406~7.322.山南土壤含水量较高,为 14.654%;拉萨最低,为 12.725%.土壤电导率表现为拉萨>山南>林芝.土壤pH与电导率表现出极显著正相关(P<0.01).3个地区土壤含有机质 9.85~16.76 g/kg、碱解氮 125.04~166.91 mg/kg、全氮 0.97~1.90 g/kg、全钾 5.37~7.08 g/kg、全磷 0.52~1.26 g/kg、速效磷 24.07~40.52 mg/kg、速效钾 127.53~371.83 mg/kg.土壤酶活性NAG为 0.58~1.05 μmol/(g·h),LAP为 0.68~0.72 μmol/(g·h),BG为 0.57~1.56 μmol/(g·h),PPO为 1.46~2.37 μmol/(g·h),POD酶活性为 2.37~3.15 μmol/(g·h).拉萨土壤酶活性与土壤有机质、电导率和pH存在正相关性,与其他指标存在负相关性.山南土壤酶活性与土壤速效磷、速效钾和含水量存在正相关性,与其他指标存在负相关性.林芝土壤酶活性与土壤全磷、全钾、全氮和碱解氮存在正相关性;与其他指标存在负相关性.[结论]拉萨、山南和林芝 3个不同地区大花黄牡丹生境土壤有机质含量总体较缺乏,全氮含量中等,全磷、全钾含量较缺乏,碱解氮、速效磷含量较丰富,速效钾含量丰富,大花黄牡丹对土壤自然肥力的要求不高.拉萨和山南地区对大花黄牡丹生境土壤酶活性影响较大的土壤环境因子为有机质和含水量,林芝地区为有机质和全钾含量.在大花黄牡丹的人工栽培过程中,需根据不同生长期调整各营养元素的配比,综合考虑多种因素,为改良牡丹野生种的土壤环境、实现优质种质资源广谱性种植奠定基础.
[Objective]The soil physical and chemical properties and enzyme activities of Paeonia ludlowii habitat were analyzed to provide a reference for the development of plateau peony industry.[Method]The water content(SM),pH,electrical conductivity(EC),organic matter(SOM),total nitrogen(TN),total phosphorus(TP),total potassium(TK),available nitrogen(AN),available phosphorus(AP),available potassium(AK),and activity of β-N-acetylglucosaminidase(NAG),β-glucosidase(BG),leucine aminopeptidase(LAP),peroxidase(POD)and polyphenol oxidase(PPO)of soil were detected with taking the soil of P.ludlowii habitat from Lhasa,Shannan and Nyingchi as the study material,and then the adaptability of P.ludlowii to soil was analyzed.[Result]The soil pH of P.ludlowii habitat from Lhasa,Shannan and Nyingchi ranged from 6.406 to 7.322.The soil water content of Shannan was higher with 14.654%,while that of Lhasa was the lowest with 12.725%.The electrical conductivity showed as Lhasa>Shannan>Nyingchi.There was extremely significant correlation between pH and electrical conductivity in soil(P<0.01).The organic matter,alkali-hydrolyzable ammonia,total nitrogen,total potassium,total phosphorus,available phosphorus and available potassium were 9.85-16.76 g/kg,125.04-166.91 mg/kg,0.97-1.90 g/kg,5.37-7.08 g/kg,0.52-1.26 g/kg,24.07-40.52 mg/kg,127.53-371.83 mg/kg,respectively.The activity of soil enzyme NAG,LAP,BG,PPO and POD were 0.58-1.05 μmol/(g·h),0.68-0.72 μmol/(g·h),0.57-1.56 μmol/(g·h),1.46-2.37 μmol/(g·h)amd 2.37-3.15 μmol/(g·h)respectively.The soil enzyme activity of Lhasa was positively correlated with organic matter,electrical conductivity and pH,but negatively correlated with other indicators.The soil enzyme activity of Shannan was positively correlated with available phosphorus,available potassium and water content of soil,but negatively correlated with other indicators.The soil enzyme activity of Nyingchi was positively correlated with total phosphorus,total potassium,total nitrogen and available nitrogen,but negatively correlated with other indicators.[Conclusion]The soils of P.ludlowii habitat from Lhasa,Shannan and Nyingchi show relatively insufficient content of organic matter on the whole,medium content of total nitrogen,relatively insuffcient total phosphorus and total potassium,relatively abundant available nitrogen and available phosphorus,abundant available potassium,and P.ludlowii has lower demand to soil fertility.Organic matter and water content are the soil environmental factor with higher impact on soil enzyme activity of P.ludlowii habitat from Lhasa and Shannan,while organic matter and total nitrogen content are the the soil environmental factor with higher impact on soil enzyme activity of P.ludlowii habitat from Nyingchi.In the process of artificial cultivation of P.ludlowii,the ratio of each nutrient element needs to be adjusted according to different growth periods,the factors should be comprehensively considered,so as to lay a foundation for improving the soil environment of wild peony species and realizing the broad-spectrum planting of high-quality germplasm resources.

peonyPaeonia ludlowiisoil physical propertiessoil chemical propertiessoil enzyme activityhabitat soilTibet

赵唯唯、张明明

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西藏大学 生态环境学院,西藏 拉萨 850000

拉萨市曲水县南木乡人民政府,西藏 曲水 850600

牡丹 大花黄牡丹 土壤物理性质 土壤化学性质 土壤酶活性 生境土壤 西藏

2024

贵州农业科学
贵州省农业科学院

贵州农业科学

CSTPCD
影响因子:0.642
ISSN:1001-3601
年,卷(期):2024.52(8)