Purification and Rejuvenation of Grain Type and Aroma of High Quality Rice Dalixiang
[Objective]The grain type and flavor of the degraded high-quality rice Dalixiang were purified and rejuvenated to provide support for the application and popularization of the excellent germplasm of Dalixiang.[Method]A total of 38 Dalixiang strains were selected as test materials in 2018,from which 6 strain materials were screened for planting in 2019 after appearance and fragrance identification,and then the initial materials were purified and rejuvenated in terms of grain type and fragrance through the determination of appearance quality,fragrance phenotypes and 2-acetyl-1-pyrroline(main aroma substance)content as well as the verification of molecular markers in the consecutive 4 years.[Result]Through the consecutive 4 years of purification and rejuvenation,a new Dalixiang line DLX-1 with large and full grains,aroma and excellent eating quality was finally obtained.The aroma gene Badh2 occurred 8 bp loss and 3 bp mutation at 7th exton.No variations or loss of Badh2 gene was observed compared with the initial materials.The relative content of 2-acetyl-1-pyrroline was up to 0.016% .Compared with the initial materials before purification and rejuvenation,the brown rice rate,milled rice rate and head milled rice rate increased by 4.63%,16.09% and 8.45% respectively.Meanwhile,the grain length increased by 3.15% while the grain width decreased by 3.53%,and the length-width ratio increased by 4.87% .The chalkiness decreased by 21.46% .[Conclusion]Aiming at the grain type and flavor of high-quality rice Dalixiang,a four-year purification and rejuvenation process was carried out to finally complete the restoration of excellent germplasm of the grain type and flavor of Dalixiang.On the basis of keeping aroma,milling yield and chalkiness are better than that of the initial materials before purification and rejuvenation,especially in restoring grain length of Dalixiang.
Dalixiangpurification and rejuvenationspecies degenerationfragrance