首页|优质稻大粒香的粒型与香味提纯复壮研究

优质稻大粒香的粒型与香味提纯复壮研究

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[目的]对种性退化的优质稻大粒香的粒型和香味进行提纯复壮,为大粒香的推广应用提供种质支撑.[方法]2018年选用大粒香品系材料38株,经外观和香味鉴定从中筛选出6个株系于2019年种植,连续 4年对外观品质、香味表型和 2-乙酰-1-吡咯啉(2-Acetyl-1-pyrroline,主要香味物质)含量检测及分子标记验证,在粒型和香味方面对初选材料进行提纯复壮.[结果]通过连续 4年提纯复壮最终获得粒大饱满、具有香味、食味品质极佳的大粒香新品系DLX-1,其香味基因Badh2在第 7外显子发生 8bp缺失和 3bp突变,与原始材料相比,Badh2基因未发生改变或丢失;2-乙酰-1-吡咯啉相对含量达 0.016%.与大粒香提纯复壮前初始材料相比,新品系DLX-1的糙米率、精米率和整精米率分别提高4.63%、16.09%和8.45%;粒长增加3.15%,粒宽降低 3.53%,长宽比增加 4.87%;垩白度降低 21.46%.[结论]针对优质稻大粒香的粒型及香味进行为期4年的提纯复壮过程,最终完成大粒香粒型和香味优良种性恢复,在保持香味基础上,出米率和垩白度均明显优于提纯复壮前初始材料,尤其在恢复大粒香长粒型特点方面表现更明显.
Purification and Rejuvenation of Grain Type and Aroma of High Quality Rice Dalixiang
[Objective]The grain type and flavor of the degraded high-quality rice Dalixiang were purified and rejuvenated to provide support for the application and popularization of the excellent germplasm of Dalixiang.[Method]A total of 38 Dalixiang strains were selected as test materials in 2018,from which 6 strain materials were screened for planting in 2019 after appearance and fragrance identification,and then the initial materials were purified and rejuvenated in terms of grain type and fragrance through the determination of appearance quality,fragrance phenotypes and 2-acetyl-1-pyrroline(main aroma substance)content as well as the verification of molecular markers in the consecutive 4 years.[Result]Through the consecutive 4 years of purification and rejuvenation,a new Dalixiang line DLX-1 with large and full grains,aroma and excellent eating quality was finally obtained.The aroma gene Badh2 occurred 8 bp loss and 3 bp mutation at 7th exton.No variations or loss of Badh2 gene was observed compared with the initial materials.The relative content of 2-acetyl-1-pyrroline was up to 0.016% .Compared with the initial materials before purification and rejuvenation,the brown rice rate,milled rice rate and head milled rice rate increased by 4.63%,16.09% and 8.45% respectively.Meanwhile,the grain length increased by 3.15% while the grain width decreased by 3.53%,and the length-width ratio increased by 4.87% .The chalkiness decreased by 21.46% .[Conclusion]Aiming at the grain type and flavor of high-quality rice Dalixiang,a four-year purification and rejuvenation process was carried out to finally complete the restoration of excellent germplasm of the grain type and flavor of Dalixiang.On the basis of keeping aroma,milling yield and chalkiness are better than that of the initial materials before purification and rejuvenation,especially in restoring grain length of Dalixiang.

Dalixiangpurification and rejuvenationspecies degenerationfragrance

王倩、吴娴、宫彦龙、张习春、王忠妮、朱速松

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贵州省水稻研究所,贵州 贵阳 550006

大粒香 提纯复壮 种性退化 香味

贵州省科技计划项目贵州省农业科学院青年科技基金项目

黔科合基础-ZK[2021]一般124黔农科院青年科技基金[2023]30

2024

贵州农业科学
贵州省农业科学院

贵州农业科学

CSTPCD
影响因子:0.642
ISSN:1001-3601
年,卷(期):2024.52(10)
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