Effects of Different Fertilization Methods on Soil Microbial Community Structure of Loquat Orchard
[Objective]The effects of different fertilization methods on soil physicochemical properties,bacterial and fungal community composition and structure in loquat orchard were explored to provide the scientific basis for rational and efficient fertilization for loquat and sustainable utilization of soil in loquat orchard.[Method]Three different fertilization treatments,including organic fertilizer combined with chemical fertilizer+water-soluble fertilizer(T1),organic fertilizer combined with chemical fertilizer(T2)and single chemical fertilizer(CK),were designed.Metagenomic sequencing technology was used to analyze the community structure and composition characteristics of soil bacteria and fungi under different fertilization methods,and combined with soil physicochemical properties,the key soil environmental factors driving the changes of bacterial and fungal communities under different fertilization methods were analyzed.[Result]Compared with CK,T1 and T2 increased soil pH,water content,organic matter,total nitrogen and available nitrogen contents,and decreased total potassium and available potassium contents in soil.The absolute dominant phyla of the three treated soil bacteria were Proteobacteria and Acidobacteria,and the absolute dominant phyla of fungi were Ascomycetes and Basidiomycetes.T1 increased the relative abundance of Proteobacteria,Actinobacteria,Chloroflexi,Gemmatimonadota,Myxococcota,Mortierellomycota and Rozellomycota,while T2 increased the relative abundance of Proteobacteria,Verrucomicrobiota,unclassified_Fungi,Rozellomycota and Mortierellomycota.T1 caused a decrease in the diversity and richness of soil bacteria and fungi,and T2 caused an increase in the diversity and richness of soil bacteria,and a decrease in the diversity and an increase in the richness of soil fungi.The principal component analysis showed that the response of fungal community structure to fertilization methods was stronger than that of bacteria.Soil pH,available phosphorus,organic matter,water content,total potassium and available potassium were the main driving factors of bacterial community change.Soil total potassium,available phosphorus,available potassium,organic matter,pH,water content and available nitrogen were the main driving factors of fungal community change.[Conclusion]Organic fertilizer combined with chemical fertilizer+water-soluble fertilizer has more advantages in improving soil fertility,increasing beneficial microorganisms quantity and enhancing plant resistance,which has greater popularization value in loquat planting areas in China.
万方数据
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