[Objective]The in vitro rapid propagation system of apple rootstock Malus hupehensis was established to provide the technical support for the annual supply of apple rootstock seedlings.[Method]The young spring shoots with bud stems of M.hupehensis were used as explants to explore the effects of different plant growth regulators(6-BA,IBA)with different concentrations on the proliferation and rooting of tissue culture seedlings,and the optimal medium formula for proliferation and rooting was screened to establish the in vitro rapid propagation system of M.hupehensis.[Result]The germination rate of young spring shoots with bud stems of M.hupehensis inoculated with the medium of MS+2 mg/L 6-BA+0.1 mg/L NAA+0.3 mg/L GA3 was 77.78% after disinfection treatment.The optimal proliferation medium was MS+1.0 mg/L 6-BA+0.1 mg/L IBA+7 g/L agar+30 g/L sucrose,and the proliferation coefficient was 8.13.The optimal rooting medium was 1/2 MS+0.45 mg/L IBA+7 g/L agar+30 g/L sucrose.The rooting rate was 97.78%,and the average root number and length were 3.28 and 3.35 cm,respectively.After 30 days of rooting culture,the transplanting survival rate of tissue culture seedlings was 85.27% .[Conclusion]The optimal proliferation medium formula for M.hupehensis is MS+1.0 mg/L 6-BA+0.1 mg/L IBA+7 g/L agar+30 g/L sucrose.The optimal rooting medium formula is 1/2 MS+0.45 mg/L IBA+7 g/L agar+30 g/L sucrose.The tissue culture rapid propagation system can effectively control the vitrification phenomenon of M.hupehensis,and improve its proliferation rate and rooting rate.
维普
万方数据