Effects of Different Selenium Concentrations on the Germination and Physiochemical Properties of Peanut Seeds
[Objective]The effects of different concentrations of selenium solution on the germination,physiological and biochemical indicators of peanut seeds were explored,and the appropriate concentration to promote the germination of peanut seeds was clarified,so as to provide a theoretical basis for the production of selenium-rich peanut sprouts.[Method]Peanut seeds were soaked in 0 μmol/L(CK),2.5 μmol/L,5.0 μmol/L,7.5 μmol/L and 10.0 μmol/L sodium selenite solutions for 12 hours.The germination ability and physiological and biochemical indicators(soluble sugar,soluble protein,reduced glutathione,MDA content,as well as SOD,POD,lipase and amylase activity)of peanut seeds were analyzed under different selenium concentrations.[Result]Compared with CK,the treatment of peanut seeds with 7.5 μmol/L sodium selenite solution significantly increased the germination rate of peanut seeds by 15.66 percent points and was significantly different from other treatments(P＜0.05).Peanut seeds had obvious changes in the physiological and biochemical indicators during 48 hours of germination.The activities of lipase and amylase increased by 25.00%and 34.53%,respectively,while the concentrations of soluble sugar and soluble protein increased by 23.75%and 19.17%.The activities of SOD and POD increased by 23.69%and 23.71%,respectively.The concentrations of reduced glutathione increased by 23.92%,while MDA decreased by 26.93%.[Conclusion]Soaking peanut seeds with 7.5 μmol/L Na2SeO3 can significantly increase the germination rate,improve the soluble sugar,soluble protein,glutathione concentrations,SOD,POD,lipase and amylase activities during 48 hours of germination,while reduce the concentration of MDA content.
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