Effects of Low Temperature Storage Time on Germination and Quality of Perilla frutescens Seeds
[Objective]The changes in vitality and quality of Perilla frutescens seeds stored at low temperature with different times were explored,which provided references for the preservation and application of P.frutescens germplasm.[Method]Taking 20 P.frutescens seeds as experimental materials,the germination rate and quality of the seeds stored at-15℃were measured regularly for 1,2 and 3 years,and then the changes in vitality and quality of P.frutescens seeds were analyzed.[Result]Compared with CK(the seeds before storing),the average germination rate of P.frutescens seeds increased by 9.99%after storing at-15℃for 1 year,while the germination rate decreased by 30%when storing for 2 years and 3 years.The oil content and linolenic acid content of seeds increased with the increase of storage time,and protein,palmitic acid,stearic acid and oleic acid contents decreased with the increase of storage time,while the linoleic acid content increased first and then decreased.The seed germination rate was significantly(P<0.05)and positively correlated with oil content,and was very significantly(P<0.01)and positively correlated with protein and linoleic acid,and was very significantly(P<0.01)and negatively correlated with linolenic acid.[Conclusion]Under-15℃,P.frutescens seeds stored for 1 year have relatively high germination rate,which can be used as germplasm resource preservation,propagation or field planting.P.frutescens seeds stored for 2-3 years have relatively good quality,which are suitable for food processing and utilization.