Effects of Different Domestication Times on Growth and Photosynthetic Physiological Characteristics of Saddle Shaped Bletilla striata Domesticated Seedlings
[Objective]The changes rules in growth and photosynthetic physiological characteristics of saddle shaped Bletilla striata domesticated seedlings were explored at different domestication times,which provided the theoretical basis for scientific and standardized cultivation of high-quality B.striata seedlings.[Method]Taking the saddle shaped B.striata Guiji No.1 as the research object,the growth indicators,biomass indicators,photosynthetic light response curve fitting indicators,chlorophyll content and chlorophyll fluorescence parameters of domesticated seedlings were measured at six different domestication times(30 days,60 days,90 days,120 days,150 days and 180 days)by Li-6400 photosynthetic determination system.The effects of different domestication times on growth and photosynthetic physiological characteristics of saddle shaped B.striata domesticated seedlings were studied.[Result]The root length,single leaf area and plant height of saddle shaped B.striata increased proportionally with the increase of domestication time,and the leaves number tended to stabilize at 4 pieces after 120 days of domestication.With the continuous increase of domestication time,B.striata domesticated seedlings were distributed more to the underground part,and the root biomass increased from 0.07 g on 30 d to 0.55 g on 180 d,significantly increasing by 6.86 times.The light saturation point of B.striata was significantly greater than the light compensation point,and the utilization rate of strong light was higher.The apparent quantum efficiency varied from 0.04 μmol/(m2·s)to 0.07 μmol/(m2·s).The chlorophyll and chlorophyll fluorescence parameters PSII,qP,ETR,Fo',Fm',and Fv/Fm of the saddle type B.striata reached their maximum values after 180 days of domestication,respectively 0.30,0.60,88.17,467.25,901.97 and 0.48,and the qN value decreased from 0.82 of 30 days of domestication to 0.73 of 180 days of domestication,significantly decreasing by 10.98%.[Conclusion]In the early stage of domestication,with the increase of domestication time,leaf photosynthetic capability continues to increase,and the photosynthetic effect is more obvious.After 180 days of domestication,the leaf photosynthetic capacity reaches the peak with strong photosynthetic potential and high productivity,the high-quality saddle shaped B.striata domesticated seedlings can be cultivated.