Effects of Different Environmental Factors on Seed Germination Characteristics of Original Plant of Miao Medicine Campanumoea j avanica
[Objective]The effects of different environmental factors on the seed germination of original plants of Miao Medicine Campanumoea javanica were explored to provide references for the wild nurturing and artificial cultivation of C.javanica.[Method]Taking the fruits and seeds of two original plants of Miao Medicine C.javanica,respectively C.javanica Bl.subsp.javanica(DH)and C.javanica Bl.(XH),as research materials,the effects of different temperatures(15℃,20℃and 25℃),shading degrees[0%(CK),30%,50%,70%,90%and 100%],drought stresses[PEG 6000 with 0%(CK),5%,10%,15%and 20%]and salt stresses[NaCl with 0 g/L(0%,CK),5 g/L(5%)and 10 g/L(10%)]on the seed germination characteristics of C.javanica were studied by indoor cultivation experiments.[Result]The seeds of original plants DH and XH were small,their morphological characteristics were not obviously different,but the surface color of DH seeds was slightly darker than that of XH.There were 678 and 766 seeds in the fruit of C.javanica Bl.subsp.javanica and C.javanica Bl.,and the 1000-seed weight was 0.108 g and 0.130 g,respectively.The two types of seeds had the highest germination rates at a temperature of 25℃,with 95.56%and 97.78%,respectively.When the shading degree was 30%,the two types of seeds had the highest germination rates,which were 90.00%and 98.89%.Drought and salt stress significantly affected seed germination,with the increase of PEG 6000 concentration and salt stress,seed germination rate,germination potential and germination index of two types of seeds gradually decreased.When the salt stress concentration was 10%,the seed germination rate was 0.Meanwhile,there was no significant difference in germination indicators of two types of seeds treated with PEG 6000≤5%.[Conclusion]The seeds of the two original plants of C.javanica are small,and there is no significant difference in seed morphological characteristics.There is a difference in surface color between the two seeds,and the surface color of C.javanica Bl.subsp.javanica seeds is slightly darker than that of C.javanica Bl.The suitable temperature for the germination of two types of seeds is 25℃.The shading degree of 30%-50%can increase the germination rate of two types of seeds.The seeds of C.javanica have a certain tolerance to drought stress,but poor tolerance to salt stress.
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