Structure and Antitumor Activity of Water-soluble Polysaccharide from Craterellus cornucopioides
One polysaccharide,named CCP Ⅰ,was isolated and purified from the sporophore of Craterellus cornucopioides.The relative molecular mass and monosaccharide composition of CCP Ⅰ were determined by using high-performance gel permeation chromatography and gas chromatography,and its spectra of IR and NMR were also investigated.The weight-average relative molecular mass of CCP Ⅰ was 16270 Da.It possessed typical infrared absorptions of polysaccharides and showed a-and β-configuration pyranoside.In addition.It was determined as a kind of heteropolysaccharide containing fucose,mannose,glucose and galactose in a molar ratio of 1∶2.11∶3.13∶6.45.Most of glycan C-6 were substituted,and part of C-2,C-3 or C-4 was replaced.Moreover,its anti-complement activity in vitro and antitumor activity by MTT were evaluated,which indicated that the anti-complement activity reached 70.44％ when the concentration of CCP Ⅰ was 4 mg/mL and it remarkably inhibited the growth of human liver cancer cell line Hep G2 and human breast cancer cell line MCF-7 in a dose-dependent manner.When the concentration ofCCP Ⅰ was 1000 μg/mL,the inhibition ratios of Hep G2 and MCF-7 were 60.06％ and 55.80％,respectively.
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