A Study of Flavonoids from the Leaves of Corylus heterophylla Fisch.
Qualitative analysis of flavonoids in the leaves of Corylus heterophylla Fisch.was performed using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS).Compounds were identified by comparison of retention times,parent ions,and secondary ion fragments.Quantitative analysis of the flavonoids was performed using HPLC-ESI-MS/MS in the multiple reactions monitoring (MRM) mode.The results showed that 12 compounds were found in the leaves of C.heterophylla,and 11 compounds were reported for the first time,namely epicatechin (41.45 ± 1.87 lμg/g,dry weight),chlorogenic acid (3-caffeoylquinic acid,389.00 ± 17.15 μg/g),syringetin-3-O-glucoside (6169.78 ± 209.73 μg/g),salvianolic acid C (443.37 ± 21.07 μg/g),chrysoeriol-6-C-glucoside (206.81 ± 10.07 μg/g),hyperoside (quercetin-3-O-galactoside,154.44 ± 7.63 μg/g),quercitrin (quercetin-3-O-rhamnoside,937.15 ± 40.86 μg/g),laricitrin-3-O-galactoside (17.27 ± 0.82 μg/g),malvidin-3-O-glucoside (202.74 ± 10.04 μg/g),laricitrin-3-O-glucoside (101.27 ± 4.99 μg/g),and kaempferol-3-O-rhamnoside (112.37 ± 4.77 μg/g).
leaves of Corylus heterophylla Fisch.flavonoidshigh-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometrycomponents
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