Phyllanthus emblica(PE)is an edible and medicinal fruit that has received much attention in recent years,but little has been reported about its anti-obesity and lipid-lowering effects as well as specific functional components.In this study,the flavonol-rich polyphenols from Yunnan wild PE were extracted(Phyllanthus emblica Extract,PEE).It was found that in the 3T3-L1 cell model,PEE could significantly inhibit the differentiation of 3T3-L1 cells into mature adipocytes and reduce the production of lipid droplets.When PEE at 300 μg/mL was applied,the relative contents of total triglyceride(TG)and total cholesterol(TC)in cells were reduced to 66.47%and 40.87%,respectively,suggesting that PEE in Yunnan wild PE has the potential to promote weight loss and lipid reduction.HPLC analysis revealed that Yunnan wild PE contained ellagic acid(3 030.78 μg/g)and fisetin(560.70 μg/g).Ellagic acid and fisetin were mixed at a 5:1 ratio to form a mixture(EF),and the effect of EF on a high-fat diet(HFD)mouse model was examined.The results showed that EF could inhibit the growth of body weight and white fat in HFD mice,and decreased the serum TG,TC and LDL-C contents by 45.48%,12.06%and 37.54%,respectively,while increasing the HDL-C level by 18.75%.The liver index,serum ALT and AST of the HFD mice decreased by 7.80%,21.59%and 41.64%,respectively.EF exhibited anti-obesity and lipid-lowering effects,and could exert certain repairing functions on the liver,thus,EF are important components accounting at least paritially for the anti-obesity and lipid-lowering effects of Yunnan wild PE.
维普
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