To study the protective effect of blueberry,blackcurrant,medlar,cassia seed complex(LHGJ)against the oxidative stress injury in human retinal epithelial cells(APRE-19)induced by methylglyoxal(MGO).The ARPE-19 cell model was established through inducing oxidative stress by MGO,and LHGJ solutions at different concentrations were used to treat the cells.Cell viability was determined by the CCK-8 method,the reactive oxygen species(ROS)content in cells was measured by the fluorescence microscopy method,the nitric oxide(NO)content was measured by enzyme-linked immunosorbent assay(ELISA),the glutathione peroxidase(GSH-PX)content was measured by colorimetry,and the superoxide dismutase(SOD)activity was measured by the hydroxylamine method.The results showed that oxidative stress injury in ARPE-19 cells could be induced by 0.80 mmol/L MGO.The induced ARPE-19 cells were then treated with 0.10 or 1.00 μg/mL LHGJ complex,which increased the cell viability by 23.03%and 21.23%,respectively(P<0.001).LHGJ complex solutions at 0.01,0.10 and 10.00 μg/mL significantly(P<0.05)reduced the fluorescence intensity of ROS(by 1.02,1.28 and 2.37 times,respectively),LHGJ complex solutions at 0.01,0.10 and 10.00 μg/mL significantly(P<0.05)decreased NO production(by 13.24,15.23 and 16.24 μmol/g prot.,respectively),LHGJ complex solutions at 1.0 and 10.00 μg/mL significantly(P<0.05)increased the activity of SOD(by 6.01 and 7.43 U/mg,respectively)(P<0.05,P<0.01)and the content of GSH-Px(by 14.32 and 25.42 U/mg,respectively)(P<0.05).LHGJ complex can protect ARPE-19 cells against oxidative stress injury induced by MGO,which provides a theoretical basis for further development of LHGJ complex.
维普
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