Protective Effect of a Complex of Blueberry,Blackcurrant,Medlar and Cassia Obtusifolia Against Oxidative Stress Injury in Retinal Epithelial Cells
To study the protective effect of blueberry,blackcurrant,medlar,cassia seed complex(LHGJ)against the oxidative stress injury in human retinal epithelial cells(APRE-19)induced by methylglyoxal(MGO).The ARPE-19 cell model was established through inducing oxidative stress by MGO,and LHGJ solutions at different concentrations were used to treat the cells.Cell viability was determined by the CCK-8 method,the reactive oxygen species(ROS)content in cells was measured by the fluorescence microscopy method,the nitric oxide(NO)content was measured by enzyme-linked immunosorbent assay(ELISA),the glutathione peroxidase(GSH-PX)content was measured by colorimetry,and the superoxide dismutase(SOD)activity was measured by the hydroxylamine method.The results showed that oxidative stress injury in ARPE-19 cells could be induced by 0.80 mmol/L MGO.The induced ARPE-19 cells were then treated with 0.10 or 1.00 μg/mL LHGJ complex,which increased the cell viability by 23.03%and 21.23%,respectively(P<0.001).LHGJ complex solutions at 0.01,0.10 and 10.00 μg/mL significantly(P<0.05)reduced the fluorescence intensity of ROS(by 1.02,1.28 and 2.37 times,respectively),LHGJ complex solutions at 0.01,0.10 and 10.00 μg/mL significantly(P<0.05)decreased NO production(by 13.24,15.23 and 16.24 μmol/g prot.,respectively),LHGJ complex solutions at 1.0 and 10.00 μg/mL significantly(P<0.05)increased the activity of SOD(by 6.01 and 7.43 U/mg,respectively)(P<0.05,P<0.01)and the content of GSH-Px(by 14.32 and 25.42 U/mg,respectively)(P<0.05).LHGJ complex can protect ARPE-19 cells against oxidative stress injury induced by MGO,which provides a theoretical basis for further development of LHGJ complex.
万方数据
维普
