In this study,Thompson seedless grapes from Turpan area were used as the experimental materials.After hot-air drying at 25℃and 30℃,the grape samples with/without browning that underwent 25%,and 50%of water loss were withdrwan.Transcriptome sequencing technology was used to screen out the key genes related to membrane lipid degradation and metabolism,and real-time fluorescence quantitative PCR technology was used for verification.The results showed that a total of 1.163 billion clean data were obtained by transcriptome sequencing.When the water loss in Thompson seedless grapes was 50%,the comparison between the non-browning and the browning species revealed 718 differentially expressed genes for the rapid dehydration group and 2 259 differentially expressed genes for the slow dehydration group.After the GO function enrichment and KEGG enrichment analyses,43 differential genes related to membrane lipid metabolism were screened,which were classified into 5 metabolic pathways.Seven key genes related to membrane lipid metabolism were screened from the obtained differential genes,including Aldehyde dehydrogenase 7B4(ALDH7B4),Digalactose diglycerol synthetase 1(DGD1),Lipoxygenase(LOX),Lipid phosphate phosphatase 2(LPP2),Diacylglycerol kinase 5(DGK5),Non-specific phospholipase C4(NPC)and Phospholipase Dα1(PLDα1).qRT-PCR verification confirmed the agreement of the gene expression trend with the transcriptome sequencing results.In conclusion,the changes of membrane lipid degradation and metabolism-related gene expression have influence on dehydration-induced browning in Thompson seedless grapes.
维普
万方数据
