Ameliorating Effects of Moringa oleifera Seed Protein Hydrolysate on Intestinal Mucositis in Mice
The ameliorating effects of Moringa oleifera seed protein hydrolysate(MSPH)on intestinal mucositis in mice were investigated.Moringa oleifera seed protein was extracted,enzymolized,and freeze-dried to obtain MSPH.Male BALB/c mice were randomly divided into the blank control(Con)group,the model(Mod)group,and the MSPH treatment group with six mice in each group.5-fluorouracil(5-FU)was intraperitoneally injected to induce intestinal mucositis in mice,and MSPH(800 mg/kg·BW)was administered orally for intervention.The weights of the mice were recorded daily.The intestinal histopathology was observed using HE staining,and the villus height/crypt depth was calculated.The concentrations of INF-γ,SIgA,ROS,G-CSF,ET,and D-LA in serum and those of IL-1β,IL-6,MDA,MPO,and TNF-α in the small intestine were determined using enzyme-linked immunosorbent assay.The mRNA expressions of ZO-1 and occludin in the small intestine were determined using real-time fluorescence quantitative polymerase chain reaction.Compared to the mice in the Mod group,the body masses of those in the MSPH group significantly increased(P=0.0011)in the later stage of the experiment,while their spleen and thymus indices increased by 16.22%and 34.69%,respectively.The villus height/crypt depth increased by 25.88%in the MSPH group.The INF-γ,ROS,G-CSF,ET,and D-LA contents decreased by 16.85%,17.50%,14.22%,8.28%,and 9.27%,respectively,while the SIgA content increased by 23.07%in the MSPH group compared to that in the Mod group.The contents of IL-1β,IL-6,MDA,MPO,and TNF-α decreased by 9.72%,11.23%,9.74%,7.29%,and 5.17%,respectively,while mRNA expressions of ZO-1 and occludin in intestinal tissues increased by 121.64%and 29.35%,respectively,in the MSPH group.Thus,5-FU-induced intestinal mucositis in mice can be ameliorated by MSPH intervention owing to reduction in inflammation,oxidative stress and intestinal permeability.
Moringa oleifera seed protein hydrolysate5-fluorouracilintestinal mucositismitigation and improvement
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