The ameliorating effects of Moringa oleifera seed protein hydrolysate(MSPH)on intestinal mucositis in mice were investigated.Moringa oleifera seed protein was extracted,enzymolized,and freeze-dried to obtain MSPH.Male BALB/c mice were randomly divided into the blank control(Con)group,the model(Mod)group,and the MSPH treatment group with six mice in each group.5-fluorouracil(5-FU)was intraperitoneally injected to induce intestinal mucositis in mice,and MSPH(800 mg/kg·BW)was administered orally for intervention.The weights of the mice were recorded daily.The intestinal histopathology was observed using HE staining,and the villus height/crypt depth was calculated.The concentrations of INF-γ,SIgA,ROS,G-CSF,ET,and D-LA in serum and those of IL-1β,IL-6,MDA,MPO,and TNF-α in the small intestine were determined using enzyme-linked immunosorbent assay.The mRNA expressions of ZO-1 and occludin in the small intestine were determined using real-time fluorescence quantitative polymerase chain reaction.Compared to the mice in the Mod group,the body masses of those in the MSPH group significantly increased(P=0.0011)in the later stage of the experiment,while their spleen and thymus indices increased by 16.22%and 34.69%,respectively.The villus height/crypt depth increased by 25.88%in the MSPH group.The INF-γ,ROS,G-CSF,ET,and D-LA contents decreased by 16.85%,17.50%,14.22%,8.28%,and 9.27%,respectively,while the SIgA content increased by 23.07%in the MSPH group compared to that in the Mod group.The contents of IL-1β,IL-6,MDA,MPO,and TNF-α decreased by 9.72%,11.23%,9.74%,7.29%,and 5.17%,respectively,while mRNA expressions of ZO-1 and occludin in intestinal tissues increased by 121.64%and 29.35%,respectively,in the MSPH group.Thus,5-FU-induced intestinal mucositis in mice can be ameliorated by MSPH intervention owing to reduction in inflammation,oxidative stress and intestinal permeability.
Moringa oleifera seed protein hydrolysate5-fluorouracilintestinal mucositismitigation and improvement
维普
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