摘要
探讨辣木籽蛋白酶解物(Moringa oleifera Seed Protease Hydrolysate,MSPH)对小鼠肠道黏膜炎的改善作用.提取辣木籽蛋白并进行酶解,冷冻干燥得到MSPH;随机将雄性BALB/c小鼠分为空白对照(Con)组、模型(Mod)组和MSPH组(800 mg/kg·BW),每组 6 只,腹腔注射 5-FU诱导小鼠肠道黏膜炎,MSPH灌胃干预.每天记录小鼠体质量;通过HE染色观察肠道组织形态学并计算绒毛高度/隐窝深度;酶联免疫法测定小鼠血清(INF-γ、SIgA、ROS、G-CSF、ET、D-LA)和小肠组织(IL-1β、IL-6、MDA、MPO、TNF-α)含量变化;实时荧光定量聚合酶链式反应检测小肠组织ZO-1 和Occludin mRNA表达量.与Mod组相比,MSPH组小鼠在实验后期体质量显著提高(P=0.001 1),脾脏和胸腺指数分别增加 16.22%和 34.69%,肠绒毛高度/隐窝深度增加 25.88%,血清中INF-γ、ROS、G-CSF、ET、D-LA含量分别降低 16.85%、17.50%、14.22%、8.28%、9.27%,SIgA含量升高23.07%,小肠组织中IL-1β、IL-6、MDA、MPO、TNF-α含量分别降低 9.72%、11.23%、9.74%、7.29%、5.17%,肠道组织ZO-1 和Occludin mRNA表达量增加 121.64%和 29.35%.因此,MSPH可以通过减轻炎症和氧化应激及降低肠道通透性改善5-FU诱导的小鼠肠道黏膜炎.
Abstract
The ameliorating effects of Moringa oleifera seed protein hydrolysate(MSPH)on intestinal mucositis in mice were investigated.Moringa oleifera seed protein was extracted,enzymolized,and freeze-dried to obtain MSPH.Male BALB/c mice were randomly divided into the blank control(Con)group,the model(Mod)group,and the MSPH treatment group with six mice in each group.5-fluorouracil(5-FU)was intraperitoneally injected to induce intestinal mucositis in mice,and MSPH(800 mg/kg·BW)was administered orally for intervention.The weights of the mice were recorded daily.The intestinal histopathology was observed using HE staining,and the villus height/crypt depth was calculated.The concentrations of INF-γ,SIgA,ROS,G-CSF,ET,and D-LA in serum and those of IL-1β,IL-6,MDA,MPO,and TNF-α in the small intestine were determined using enzyme-linked immunosorbent assay.The mRNA expressions of ZO-1 and occludin in the small intestine were determined using real-time fluorescence quantitative polymerase chain reaction.Compared to the mice in the Mod group,the body masses of those in the MSPH group significantly increased(P=0.0011)in the later stage of the experiment,while their spleen and thymus indices increased by 16.22%and 34.69%,respectively.The villus height/crypt depth increased by 25.88%in the MSPH group.The INF-γ,ROS,G-CSF,ET,and D-LA contents decreased by 16.85%,17.50%,14.22%,8.28%,and 9.27%,respectively,while the SIgA content increased by 23.07%in the MSPH group compared to that in the Mod group.The contents of IL-1β,IL-6,MDA,MPO,and TNF-α decreased by 9.72%,11.23%,9.74%,7.29%,and 5.17%,respectively,while mRNA expressions of ZO-1 and occludin in intestinal tissues increased by 121.64%and 29.35%,respectively,in the MSPH group.Thus,5-FU-induced intestinal mucositis in mice can be ameliorated by MSPH intervention owing to reduction in inflammation,oxidative stress and intestinal permeability.
基金项目
国家自然科学基金(31960508)
国家木薯产业技术体系建设项目(CARS-11-YNTY)
云南省重大科技专项绿色食品国际合作研究中心项目(2019ZG00905)
云南省科技重大专项(202002AA100005)
维普
万方数据