摘要
通过建立慢性轻度不可知应激刺激(CUMS)诱导的小鼠抑郁模型,研究当归挥发油对抑郁行为的影响及机制.小鼠随机分为正常组、模型组、低、中、高剂量当归挥发油组(15、30、60 mg/kg)及盐酸氟西汀组(2.1 mg/kg).干预4周后发现,与模型组比较,当归挥发油各剂量组小鼠中央穿越次数、中央区活动时间及糖水偏好度升高(P<0.01),悬尾不动时间下降(P<0.05,P<0.01),海马CA1 区病理变化减轻,血清白细胞介素-1β(IL-1β)、IL-6 及肿瘤坏死因子-α(TNF-α)含量降低(P<0.01),而IL-10 含量增加(P<0.01);除低剂量当归挥发油组小鼠脑组织多巴胺(DA)含量及神经营养蛋白-3(NT-3)表达无明显变化外(P>0.05),剩余各组小鼠DA、去甲肾上腺素(NE)、5-羟色胺(5-HT)含量及神经生长因子(NGF)、NT-3、脑源性神经营养因子(BDNF)表达均升高(P<0.05,P<0.01).上述结果表明,当归挥发油具有改善CUMS诱导的小鼠抑郁行为作用,该作用与抑制神经炎症、上调单胺类神经递质含量及促进神经营养因子表达有关.
Abstract
To determine the effects and mechanism of Angelica sinensis volatile oil(ASVO)on depressive behavior,chronic unpredictable mild stress(CUMS)-induced depression mouse models were established.Mice were divided into normal,model,low-dose,medium-dose,and high-dose ASVO(15,30,and 60 mg/kg,respectively),and fluoxetine hydrochloride(2.1 mg/kg)groups.After four weeks of intervention,the number of central zone crossing,central zone activity duration,and sucrose preference were increased(P<0.01)while the tail suspension time was reduced(P<0.05,P<0.01)in all ASVO groups compared with those in the model group.The pathological changes in the hippocampal CA1 region were alleviated,and the contents of serum interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)decreased(P<0.01)while that of IL-10 increased(P<0.01).Despite no significant changes in the dopamine(DA)content and neurotrophin-3(NT-3)expression in the brain tissue of mice in the low-dose ASVO group(P>0.05),the contents of DA,norepinephrine(NE),and 5-hydroxytryptamine(5-HT)and the expression of nerve growth factor(NGF),NT-3,and brain-derived neurotrophic factor(BDNF)increased in the other groups(P<0.05,P<0.01).These results indicate that ASVO can improve CUMS-induced depressive behavior in mice,which is related to the inhibition of neuroinflammation,upregulation of monoamine neurotransmitter contents,and promotion of neurotrophic factor expression.
基金项目
吉林省教育厅科技项目(JJKH20210059KJ)
吉林省教育厅科技项目(JJKH20210055KJ)
维普
万方数据