Identification of pork with lymphoid tissues based on quantitative analysis of inflammation and immunity-related metabolites through ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was studied.Samples were extracted with 80%acetonitrile-water and degreased with n-hexane.The separation was carried out using a BEH C18 column(2.1 mm×100 mm,1.7 μm),with 5 mmol/L ammonium acetate solution-acetonitrile as the mobile phase.Gradient elution was conducted for 25 min,and positive and negative ions were determined simultaneously.A quantitative method for 38 inflammation and immunity-related metabolites based on UPLC-MS/MS was established to quantitatively examine these metabolites in ordinary pork and pork with lymphoid tissues,and the identification model for the latter was constructed by chemometrics.The results reveal a good linear relationship between the target compounds in the concentration range of interest.The recoveries of low concentration spiked samples range from 50.3%to 118.8%,and the recoveries of high concentration spiked samples range from 57.0%to 127.0%,with RSDs below 20%.Based on the PCA and OPLS-DA models,the pork samples with lymphoid tissues and ordinary pork samples can be well identified,and the samples with lymphoid tissue proportions more than 20%can be distinguished from ordinary samples.The R2 and Q2 were 0.954 and 0.858,respectively,and the model showed no over-fitting and good prediction performance.The contents of inflammation and immunity-related metabolites combined with chemometrics can effectively distinguish pork samples with lymphoid tissues from ordinary pork samples,providing a reliable method for the identification of pork with lymphoid tissues.
pork with lymphoid tissuesimmunityinflammationchemometricsfood fraud
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