An internal standard HPLC method was established for simultaneous determination of 15 kinds of PAHs in spices by.The samples were dissolved in sodium hydroxide,extracted with acetonitrile,redissolved by n-hexane,cleaned and concentrated by the Florisil SPE cartridge,and determined by HPLC,with benzo[a]pyrene D12 being used as the internal standard.This method had a good linear relationship in the range of 0～100 ng/mL,with the detection limits of PAHs in Capsicum being 0.5～3.0 μg/kg and the limits of quantitation as 2.0～10.0 μg/kg.The limits of detection and limits of quantitation of PAHs in Zanthoxylum were 0.5～1.0 μg/kg and 2.0～3.0 μg/kg,respectively.When the spiked levels were 2.0,10.0 and 50.0 μg/kg,the recoveries in the two spices were 60.58%～119.48%and 60.46%～119.24%,with the RSD(n=6)being 1.25%～9.61%and 1.10%～8.56%.The concentration of ∑PAHs in the five spices from high to low were:green Zanthoxylum(561.2 ųg/kg)>erjingtiao(448.8 μg/kg)>red Zanthoxylum(358.3 μg/kg)>shizhuhongi(353.6 μg/kg)>chilli(243.5 μg/kg).The more seriously polluted areas of Chongqing were Bishan,Jiangjin and Rongchang,with ∑PAHs being 721.5,626.6 and 621.0 μg/kg,respectively.The more seriously polluted compounds in PAHs were phenanthrene,fluoranthrene and pyrene,with their average contents being 215.10,80.25 and 75.35 μg/kg,respectively.The internal standard method established in this study has high accuracy and good repeatability,which is suitable for the determination of PAHs in spices.
Polycyclic Aromatic Hydrocarbons(PAHs)pollutantinternal standard methodhigh performance liquid chromatographyspices
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