萝卜硫苷的高效纯化技术研究进展
Research Progress on the Efficient Purification of Glucoraphanin
李金旺 1甄少波 2李修德 1周麟依 1刘奕忍 3李金峰4
作者信息
- 1. 北京工商大学食品与健康学院,北京 100048
- 2. 中国劳动关系学院酒店管理学院,北京 100048
- 3. 北京市科学技术研究院分析测试研究所(北京市理化分析测试中心),北京 100094
- 4. 青岛儒商饮料有限公司,山东青岛 266418
- 折叠
摘要
萝卜硫苷(Glucoraphanin,RAA)是广泛存在于十字花科植物中的硫代葡萄糖苷,其代谢产物具有防癌抗癌等功能.但从十字花科植物中提取的RAA纯度较低,降低了其利用率,因此需要对RAA纯化.当前纯化RAA的主要技术为色谱纯化技术,但该技术存在回收率低以及二次污染等问题.此外,因膜纯化技术是分离纯化糖苷类物质最常用的方法,具有设备和操作简单以及无二次污染等特点,故该技术同样是纯化RAA的潜在技术.因此,色谱纯化与膜纯化联合技术可实现RAA的有效回收并提高RAA的纯度,有效规避色谱纯化技术所造成的RAA回收率低及二次污染的缺点.因此,该文主要讨论了目前RAA主要的纯化方法,分析了色谱和膜纯化技术在RAA纯化中联合应用的可行性,为RAA的高效纯化提供方法参考.
Abstract
Glucoraphanin(RAA)is a glucosinolate widely found in cruciferous plants,the metabolites of which have been established to have anti-cancer effects.However,the purity of RAA extracted from cruciferous plants is low,which limits its effective utilization,and consequently,it is necessary to develop an effective process for RAA purification.Although the main technique currently used for the purification of RAA is chromatographic purification,this procedure has notable drawbacks,including low recovery rates and secondary contamination.As an alternative procedure,membrane purification,which is the most commonly used method for the separation and purification of glycosides,requires only simple equipment and entails simple operation.Moreover,it is less prone to secondary contamination.Accordingly,this separation procedure is considered a potential technique for the purification of RAA.It is speculated that combined chromatographic and membrane purification could facilitate the effective recovery of RAA and enhance the purity of the recovered product,thereby overcoming the problems of low recovery and secondary contamination of RAA associated with the sole use of chromatographic purification.This study focuses mainly on the main methods currently used for the purification of RAA and assesses the feasibility of the combined application of chromatography and membrane purification techniques for RAA purification,thereby providing a methodological reference for the efficient purification of RAA.
关键词
萝卜硫苷/十字花科植物/高效纯化/色谱纯化技术/膜纯化技术Key words
glucoraphanin/cruciferous plant/efficient purification/chromatographic purification technology/membrane purification technology引用本文复制引用
基金项目
国家自然科学基金青年基金项目(32302104)
中国劳动关系学院校级教改项目(JG23016)
出版年
2024
国家科技期刊平台
NSTL
万方数据