The chemical composition and antitumor activity of ethyl acetate extracts from Ganoderma lucidum compounds(GCE)were investigated.GCE was extracted using ethyl acetate from compounds of Ganoderma lucidum,Cuscuta chinensis Lam,Schisandra chinensis,and Codonopsis pilosula(at a mass ratio of 50.3:25.2:15.7:8.8).Ultra-high pressure liquid chromatography coupled with time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was used to analyze its composition.Blank,model,positive control,and GCE-treated groups(low,medium,and high dosages)were established.The tumor inhibition rate,organ index,splenic lymphocyte proliferation capacity,and serum cytokine levels were measured.The results showed that GCE is mainly composed of 11 compounds:L-pyroglutamic acid,DL-leucine adenosine,D-erythronolactone,L-norleucine,pyrogallol,2-hydroxy-3-methylpyran-4-one,chlorogenic acid,3-(3,4,5-trimethoxyphenyl)propionic acid,quercetin,and schisandrin.The tumor inhibition rates of H22 tumor-bearing mice in low-,medium-,and high-dose GCE-treated groups(at 50,100 and 200 mg/kg)H22 were 35.14%,38.25%,and 50.87%,respectively.Comparison of the model and treated groups revealed that low-,medium-,and high-dose GCE significantly promoted the proliferation of splenic lymphocytes(P<0.01).A high dose of GCE could significantly promote the secretion of IL-6,TNF-α,and IL-12 in mice(P<0.05).In short,GCE has certain inhibitory effects on the tumor of H22 tumor-bearing mice.The results of this study can provide insights into the development of antitumor products.
ethyl acetate extracts from Ganoderma lucidum compoundschemical compositionmouse modelantitumor
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