In this paper,ammonium molybdate,eggshell and cetyltrimethyl ammonium bromide were used as raw materials to prepare CaMoO4 with improved performance by adjusting the calcination temperature.Additionally,a CuO/CaMoO4 composite antibacterial agent was synthesized using calcium molybdate,copper sulfate,and sodium borohydride as raw materials.The effects of CuO/CaMoO4 antibacterial dosage,culture time,and copper content on antibacterial activity and the antibacterial effect of CuO/CaMoO4 on Escherichia coli(E.coli)were studied under light and dark reaction conditions.The CuO/CaMoO4 composite antibacterial agent synthesized at 650℃exhibited the best antibacterial efficacy.Moreover,when the copper content was 1.20%,the antibacterial rate could reach 100%under light and dark conditions.Bacteriostatic testing of plastic wrapping material containing CuO/CaMoO4 compound antibacterial agent showed that the agent could effectively inhibit E.coli on food surfaces,and the antibacterial rate remained at 100%even after four days of storage.Furthermore,the CuO/CaMoO4 antibacterial compound displayed excellent biocompatibility.Overall,the compound CuO/CaMoO4 antibacterial agent demonstrated rapid and efficient inhibition of E.coli,thereby ensuring food safety and offering novel insights for food storage practices.