Exploring Protective Effect of Phellinus linteus Polysaccharides on Liver Injury in Exercise Fatigue Mice Based on Nrf2 Signaling Pathway
Ultrasound-assisted hot water extraction was utilized to obtain Phellinus linteus polysaccharides(PLPs),and its protective effect on liver injury in exercise-induced fatigue mice was investigated through modulation of the Nrf2 signaling pathway.Results showed a PLP extraction yield of 7.64%,with a purification efficiency of 23%.PLP administration led to a significant reduction in the liver coefficient of mice compared to the exercise-induced fatigue model group.Moreover,it increased swimming endurance times of the low-,medium-and high-dose groups by 15.58%,34.69%and 53.06%,respectively.Compared to the sedentary control group,the model group exhibited significantly diminished activities of antioxidative enzymes in the liver,including superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px),alongside increased malondialdehyde(MDA)content;serum inflammatory factors such as tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β)and interleukin-6(IL-6);and alanine transaminase(ALT)and aspartate transaminase(AST)activities.Conversely,the low-,medium-and high-dose groups demonstrated elevated antioxidant enzymes activity in the liver and reduced MDA content in comparison to the model group.Notably,the high-dose group exhibited significant reductions in serum TNF-α,IL-1β,and IL-6 levels by 50.54%,50.51%,and 46.21%,respectively,alongside ALT and AST activities by 33.30%and 34.57%,respectively.Moreover,mRNA expression levels of Nrf 2,HO-1,and NQO1 were significantly lower in the model group compared to the sedentary group,while Keap1 mRNA expression was significantly higher.Post-PLP supplementation,mRNA expression levels of Nrf 2,HO-1 and NQO1 significantly increased in the low-,medium-and high-dose groups,while Keap1 mRNA expression significantly decreased.These findings collectively suggest that PLPs enhance hepatic antioxidant capacity and reduce inflammatory factors in exercise-induced fatigue mice by activating the hepatic Nrf2 signaling pathway,thereby mitigating liver injury.
万方数据
维普
