Quercetin Ameliorates Hepatic Steatosis and Influences Tryptophan Metabolites in Obese Mice Induced by a High-fat Diet
The purpose of this study was to investigated the ameliorating effect of quercetin on liver steatosis and its influence on tryptophan metabolites in obese mice induced by a high-fat diet.The obese mouse model was established using a high-fat diet.Male C57BL/6J mice were randomly divided into a control group(NC),a model group(HFD)and a quercetin intervention group(HFD+Q),with 10 mice in each group.The mice in the HFD+Q group were given 50 mg/kg.BW quercetin by gavage for 16 weeks.The body weights and liver weights of mice were measured.Fat deposition in the liver was detected tissue sections and oil red O staining.The ALT,AST,TC and TG levels were determined by ELISA.The relative expression levels of inflammatory cytokines,IL-1β,TNF-α and IL-6,and lipid metabolism-related genes,FAS,SREBP-1c and ABCA1,in the liver were determined by qPCR.The contents of serum tryptophan metabolites,indole-3-acetic acid(IAA)and indole-3-propionic acid(IPA),were determined by LC-MS targeted analysis.Quercetin intervention significantly(P＜0.05)decreased the HFD-induced body weight gain,liver steatosis,and the levels of IL-6(50.88%),IL-1β(42.52%),TNF-α(62.96%),FAS(80.36%)and SREBP-1c(23.54%),whilst increasing the mRNA expression level of ABCA1(5.21 times)and the contents of tryptophan metabolites,indole-3-acetic acid(1.71 times)and indole-3-propionic acid(1.12 times).In conclusion,quercetin can reduce liver fat deposition,decrease the expression of inflammatory factors in the liver,ameliorate HFD-induced hepatic steatosis in obese mice,while increasing the contents of serum tryptophan metabolites such as indole-3-acetic acid and 3-indolepropionic acid.
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