Roselle was used as a raw material for extracting anthocyanins.The anthocyanins were purified using Amberlite XAD-7 macroporous resin.Purified roselle anthocyanins(PRA)were identified using Fourier-transform infrared spectroscopy and ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.The antioxidant activity of PRA in vitro and in HepG2 cells was evaluated.The results showed that the anthocyanin content in PRA increased by 6.35-fold,reaching 175.27 mg/g.PRA contained five anthocyanins,of which delphinidin-3-sambubioside was the most abundant,accounting for 57.35%(m/m)of the total anthocyanins.PRA exhibited high antioxidant activity in vitro.The DPPH scavenging rate reached 90%when the PRA concentration was 0.23 mg/mL,and the hydroxyl radical scavenging rate was 97.85%at a PAR concentration of 2.0 mg/mL.Additionally,PRA showed high intracellular antioxidant activity in HepG2 cells.Treatment with 200 µg/mL PRA significantly reduced the H2O2-induced HepG2 intracellular reactive oxygen species level and nitric oxide content from 117.47%(taking the 2′,7′-dichlorofluorescein fluorescence intensity of the control group as 100%)and 53.18 nmol/mL to 102.09%and 45.79 nmol/mL,respectively.PRA treatment significantly increased the superoxide dismutase and catalase activities from 18.19 and 10.10 U/mg prot to 35.05 and 19.38 U/mg prot,respectively.The main component of PRA anthocyanins was delphinidin-3-sambubioside,which exhibited strong antioxidant activity in vitro and anti-oxidative stress response effects in vivo.These results provide a theoretical basis for the high-value development and the potential health benefits of roselle anthocyanins.
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