咖啡蜜在不同色谱柱上挥发性成分测定与对比分析
Determination and Comparative Analysis of Volatile Compounds of Coffee Honey Using Different Chromatographic Columns
荆津 1祖铁红 2孙会媛 2管志斌 3贾光群 2李阿丹 4崔宗岩2
作者信息
- 1. 燕山大学环境与化学工程学院,河北秦皇岛 066004;秦皇岛海关技术中心,河北秦皇岛 066004
- 2. 秦皇岛海关技术中心,河北秦皇岛 066004
- 3. 西双版纳金棕生物科技有限公司,云南景洪 666100;中国医学科学院药用植物研究所云南分所,云南景洪 666100
- 4. 燕山大学环境与化学工程学院,河北秦皇岛 066004
- 折叠
摘要
为了探究咖啡蜜挥发性成分及其在不同色谱柱上挥发性成分的差异,采用顶空固相微萃取-气相色谱-质谱联用技术对 19 批咖啡蜜的挥发性成分进行检测,并分析了咖啡蜜在DB-5MS和DM-Wax色谱柱上挥发性成分的差异.结果表明:咖啡蜜中共检测出 40种挥发性成分,主要包括醇类、萜烯类、醛类、酮类等,含量较高的化合物为顺式芳樟醇氧化物、脱氢芳樟醇、苯乙醛和反式芳樟醇氧化物.在DM-Wax色谱柱上检测到 40 种挥发性化合物,而在DB-5MS色谱柱上仅检测到30种.两种色谱柱上检测到的醇类物质分别为16种和10种,2种酸类物质仅在DM-Wax色谱柱上检出.总体而言,DM-Wax色谱柱对醇类和酸类物质的保留效果更好.研究结果可以为蜂蜜挥发性成分检测中色谱柱的选择提供借鉴参考,同时为咖啡蜜风味和品质研究提供技术支撑.
Abstract
To investigate the volatile components of coffee honey and their profiling differences caused by different chromatographic columns,the volatile compounds of 19 batches of coffee honey were detected by headspace solid-phase microextraction-gas chromatography-mass spectrometry,and the differences in the detected volatile compounds of coffee honey caused by DB-5MS and DM-Wax chromatographic columns were analyzed.The results showed that a total of 40 volatile compounds were detected in coffee honey,mainly including alcohols,terpenes,aldehydes,ketones,etc.The compounds with higher contents were cis-Linalool-oxide,hotrienol,benzeneacetaldehyde and trans-linalool-oxide.A total of 40 volatile compounds were detected when the DM-Wax column was used,whilst only 30 volatile compounds were detected when the DB-5MS column was used.16 and 10 alcohols were detected through using the two columns,respectively,and 2 acids were detected only when the DM-Wax column was used.In general,the DM-Wax chromatographic column exhibited a better retention effect on alcohols and acids.The results of this study can provide a reference for the selection of chromatographic columns in the determination of volatile components in coffee honey,while offering technical support for the study of coffee honey's flavor and quality.
关键词
咖啡蜜/挥发性成分/顶空固相微萃取/气相色谱-质谱法/色谱柱Key words
coffee honey/volatile compounds/headspace solid phase microextraction/gas chromatography-mass spectrometry/chromatographic column引用本文复制引用
基金项目
海关总署科研项目(2022HK023)
云南省张进杰专家工作站(202205AF150072)
河北省自然科学基金面上项目(B2021203005)
出版年
2024
国家科技期刊平台
NSTL
万方数据