Preparation,Characterization and Performance Evaluation ofα-Mangostin/Glycyrrhizic Acid Nanomicelles
α-Mangostin/glycyrrhizic acid nanomicelles(α-MG/GA NMs)were prepared to improve the solubility and antioxidant activity of α-mangostin(α-MG).Glycyrrhizic acid(GA)was used as the carrier to prepare α-MG/GA NMs,and single factor design and response surface methodology were adopted to optimize the preparation process.The characteristics,in vitro release,and in vitro antioxidant activity of α-MG/GA NMs were evaluated.The results show that the solubility of α-MG in α-MG/GA NMs prepared under the optimal conditions reached 232.65 μg/mL,which is nearly 1163 times higher than that in water.The particle size of α-MG/GA NMs was 123.46 nm,and their PDI and zeta potential were 0.24 and-20.94 mV,respectively.Transmission electron microscopy(TEM)and scanning electron microscopy(SEM)showed that the NMs are spherical.After freeze-drying,the α-MG/GA NMs prepared were stable under irradiation at high temperature and high humidity.Fourier transform infrared spectroscopy(FT-IR)showed that hydrogen bonding interactions between α-MG and GA may take place.The in vitro release analysis demonstrated that α-MG/GA NMs can promote the release of α-MG and have a sustained release effect.The cumulative release rates of α-MG/GA NMs were 47.03％and 40.56％in release media with pH values of 7.4 and 5.0,respectively,for 24 h.Compared with that of free α-MG,the antioxidant activity of α-MG/GA NMs was more substantial.Furthermore,GA and α-MG had a synergistic antioxidant effect.The synergistic indexes(CI values)of DPPH and ABTS+radical scavenging were 0.62 and 0.58,respectively.The α-MG/GA NMs prepared in this study improved the solubility and antioxidant activity of α-MG,providing a theoretical basis for applying α-MG in food,medicine,cosmetics,and other fields.
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