In this study,the growth status and active components such as adenosine(AD),polysaccharide(PS)and cordycepic acid(CA)of Cordyceps guangdongensis cultured at different mass concentrations of exogenous selenium(Na2SeO3)were investigated.The optimal mass concentration of selenium for enrichment in Cordyceps guangdongensis was determined,and the total content of selenium and its existing form in the fruiting body obtained at the optimal selenium mass concentration were examined.Meanwhile,the in vitro antioxidant activity of the polysaccharide from the fruit body was preliminarily investigated.The results indicated that,with the increase of the mass concentration of Na2SeO3 in the medium,the biological conversion rate,and the contents of AD,PS and CA in the selenium-enriched Cordyceps guangdongensis fruit body showed a trend of an initial increase followed by decrease.When the mass concentration of Na2SeO3 was 30 mg/kg,the biological conversion rate,AD content,and PS content all reached the highest values,indicating that this was the optimal mass concentration.In this case,the proportion of organic selenium was 100％,and there were 7 forms of selenium,namely SeMet,SeCys2,Se(Ⅳ),SeMeCys,Se(Ⅵ),and two unknown forms of selenium.Compared to the Cordyceps guangdongensis crude polysaccharide(CPS),the Se-enriched Cordyceps guangdongensis crude polysaccharide(CPS-Se)had higher in vitro antioxidant activities,i.e.the scavenging abilities towards the DPPH,ABTS+and hydroxyl free radicals,the total reducing ability,and the total antioxidant ability were enhanced to different extents(P＜0.01),showing good dose-dependence.This study provides a theoretical basis for the development of selenium-enriched Cordyceps guangdongensis products,and for further purification,identification,and activity verification of its polysaccharides.
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