Determination of Illegally Added Sodium Picosulfate in Internet-famous Weight-loss Foods by Salting-out Assisted and Pass-through Cleanup SPE-UHPLC-MS/MS
An ultra high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS)method was developed for the determination of illegally added sodium picosulfate in the intemet-famous weight-loss foods.The samples were extracted by a salting-out assisted methanol-water solution system,and purified by an Oasis PRiME HLB solid phase extraction column with a pass-through cleanup strategy.The separation was carried out on a C18 column using acetonitrile-ammonium acetate as the mobile phase in a gradient elution mode.Eletrospray ionization source with positive ion(ESI+)ionization mode,multiple reaction monitoring(MRM)scanning and external standard quantification method were used.In this study,the extraction system was optimized to achieve good dispersion of different matrix samples and good anti-interference effect.The established method showed a linear relationship in the picosulfate concentration range of 0.50～250.00 ng/mL,with the correlation coefficients higher than 0.998,and the limit of detection(LOD)and the limit of quantitation(LOQ)being 10.00 μg/kg and 25.00 μg/kg,respectively.At low(25.00 μg/kg),medium(50.00 μg/kg)and high(250.00 μg/kg)concentrations,the average recoveries ranged from 81.51％to 104.53％,with the relative standard deviation(RSD)ranging from 1.14％to 5.64％.This method is somple,efficient and accurate with strong anti-interference,thus is suitable for the qualitative and quantitative analyses of sodium picosulfate illegally added to weight-loss foods.
diet foodsodium picosulfatesalting outpass-throughultra high performance liquid chromatography-tandem mass spectrometry
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