The probiotic effects of Bifidobacterium animalis subsp.lactis XLTG11 on the intestinal tract of mice were investigated.After adaptive feeding for seven days,48 BALB/c mice were randomly divided into a blank control group,XLTG11 low dose group,XLTG11 middle dose group,and XLTG11 high dose group.The three subject groups had B.animalis Lactis subspecies XLTG11 bacterial powder gavage,with doses of 1.6×106 CFU/each,1.6×107 CFU/each,and 1.6x108 CFU/each,respectively.The gavage of the blank control group had an equal volume of saline.After 14 days of gavage,the body mass,intestinal microbiota,histopathology,intestinal barrier,intestinal permeability,and short-chain fatty acid content of mice were measured.The results indicated that,compared to the blank control group,the body mass of mice in the high-dose group increased by 14.51％.Changes occurred to the intestinal microbiota abundance and composition,with the numbers of Bifidobacterium and Lactobacillus in the feces of mice increasing by 29.32％and 33.88％,respectively.The quantity of Clostridium perfringens decreased by 24.49％,and the expression level of tight junction proteins and mucins increased in the low-,medium-,and high-dose groups.The levels of LPS and D-lactic acid in the sera of mice also decreased.These findings indicated that B.animalis subsp.lactis XLTG11 could enhance intestinal barrier function and reduce intestinal permeability.In addition,the levels of short-chain fatty acids in the cecum of mice in the three groups were 13.66％,43.04％,and 48.58％,respectively.In conclusion,this study demonstrated that B.animalis subsp.lactis XLTG11 could confer health benefits to the intestine in mice and had a regulatory and ameliorative effect on the intestinal microbiota.
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