Analysis on Antioxidant Activity and Hepatoprotective Effects of Cynara Scolymus L.-Based Herbal Formula
Zebrafish studies were conducted to test the in vitro antioxidant,anti-inflammatory activity,and hepatoprotective effects of the combined herbal formula of Cynara scolymus L.and the three medicine-food homology plants(Hovenia dulcis.,Pueraria lobata,and Curcuma longa).The in vitro anti-oxidative activity showed that the scavenging capacity values,IC50,of 1-diphenyl-2-trinitrobenzene hydrazine(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)free radicals were 80.18 μg/mL and 256.15 μg/mL,respectively.The in vitro anti-inflammatory assay showed that the hepatoprotective formula significantly inhibited expression levels and secretion(P＜0.05)of the inflammation factors,TNF-α,IL-6,and NO,with inhibition rates of 48.12％,31.77％,and 27.38％,respectively.The results of acetaminophen(APAP)-induced liver injury in zebrafish showed that liver areas increased by 30.07％and liver brightness significantly improved by 12.48％,.The vitelline sac areas significantly decreased by 56.16％in the high-dose group(62.5 μg/mL)(P＜0.001).The hepatocellular degeneration and cell necrosis were significantly improved in the low-dose,middle-dose,and high-dose groups(15.6,31.25,and 62.5 μg/mL,respectively).In the liver tissue,the alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activities decreased(P＜0.05),and showed a dose-dependent relationship.The result of this study showed a Cynara scolymus L.-based hepatoprotective herbal formula exhibited significant hepatoprotective effects on APAP-induced liver damage in zebrafish,presenting strong in vitro antioxidant activity and anti-inflammatory activity.
万方数据
维普
