摘要
探究朝鲜蓟和3种药食同源中草药枳椇子、葛根和姜黄联用下的体外抗氧化和抗炎活性及其对斑马鱼肝损伤的保护作用.体外抗氧化研究表明,护肝组方对DPPH和ABTS+自由基的清除能力IC50值分别为80.18 μg/mL和256.15 μg/mL.体外抗炎实验研究表明,质量浓度为120 μg/mL的护肝组方显著抑制了炎症因子TNF-α、IL-6的表达和NO的分泌(P＜0.05),抑制率分别为48.12％、31.77％和27.38％.对乙酰氨基酚(APAP)致斑马鱼肝损伤研究表明,高剂量组(62.5 μg/mL)中斑马鱼肝脏面积增加了 30.07％,肝脏明亮度增加了 12.48％,卵黄囊面积降低了 56.16％,差异均显著(P＜0.001).低、中、高剂量组(15.6、31.25及62.5 μg/mL)均显著改善肝细胞变性和细胞坏死并降低组织中谷丙转氨酶(ALT)和谷草转氨酶(AST)活力(P＜0.05),且呈剂量依赖关系.该研究表明,朝鲜蓟护肝组方具有明确的体外抗氧化和抗炎活性,对APAP所致斑马鱼肝损伤具有明显的保护作用.
Abstract
Zebrafish studies were conducted to test the in vitro antioxidant,anti-inflammatory activity,and hepatoprotective effects of the combined herbal formula of Cynara scolymus L.and the three medicine-food homology plants(Hovenia dulcis.,Pueraria lobata,and Curcuma longa).The in vitro anti-oxidative activity showed that the scavenging capacity values,IC50,of 1-diphenyl-2-trinitrobenzene hydrazine(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)free radicals were 80.18 μg/mL and 256.15 μg/mL,respectively.The in vitro anti-inflammatory assay showed that the hepatoprotective formula significantly inhibited expression levels and secretion(P＜0.05)of the inflammation factors,TNF-α,IL-6,and NO,with inhibition rates of 48.12％,31.77％,and 27.38％,respectively.The results of acetaminophen(APAP)-induced liver injury in zebrafish showed that liver areas increased by 30.07％and liver brightness significantly improved by 12.48％,.The vitelline sac areas significantly decreased by 56.16％in the high-dose group(62.5 μg/mL)(P＜0.001).The hepatocellular degeneration and cell necrosis were significantly improved in the low-dose,middle-dose,and high-dose groups(15.6,31.25,and 62.5 μg/mL,respectively).In the liver tissue,the alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activities decreased(P＜0.05),and showed a dose-dependent relationship.The result of this study showed a Cynara scolymus L.-based hepatoprotective herbal formula exhibited significant hepatoprotective effects on APAP-induced liver damage in zebrafish,presenting strong in vitro antioxidant activity and anti-inflammatory activity.
基金项目
云南特色植物提取实验室自主研究项目基金(2022YKZY005)
维普
万方数据