现代食品科技2024,Vol.40Issue(9) :270-277.DOI:10.13982/j.mfst.1673-9078.2024.9.1042

脉冲电场强化生姜姜酚提取及工艺优化

Enhanced Extraction of Ingerol from Ginger Using Pulsed Electric Fields and Process Optimization

李晓娟 钟梨 彭开锋 尹军 曾新安 刘志伟 韩忠
现代食品科技2024,Vol.40Issue(9) :270-277.DOI:10.13982/j.mfst.1673-9078.2024.9.1042

脉冲电场强化生姜姜酚提取及工艺优化

Enhanced Extraction of Ingerol from Ginger Using Pulsed Electric Fields and Process Optimization

李晓娟 1钟梨 2彭开锋 1尹军 1曾新安 3刘志伟 4韩忠2
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作者信息

  • 1. 株洲千金药业股份有限公司,湖南株洲 412000
  • 2. 华南理工大学食品科学与工程学院,广东广州 510640
  • 3. 华南理工大学食品科学与工程学院,广东广州 510640;佛山科技学院广东省食品智能制造重点实验室,广东佛山 528225
  • 4. 湖南农业大学食品科学技术学院,湖南长沙 410128
  • 折叠

摘要

脉冲电场(PEF)作为一种新型的物理场非热加工技术,其在流体食品的杀菌、灭酶等方面已开展大量的应用研究.近年来,基于PEF"电穿孔"对细胞优越的破壁效果,其在细胞的穿孔、强化胞内功能性成分的提取已逐渐成为研究热点.该文以生姜为对象,首先对比了不同提取工艺[传统溶剂浸提法(TET)、超声辅助提取法(UET)、索氏提取法(SET)、脉冲电场辅助提取法(PEF)]对生姜姜酚提取量的影响,然后利用响应面法优化了 PEF强化生姜姜酚的提取参数.结果表明,与TET、UET、SET相比,PEF处理对生姜姜酚提取量最高,达13.36 mg/g·DW.进一步通过单因素和响应面试验设计,获得的最佳工艺参数为电场强度:2.0kV/cm,脉冲次数:22个,脉冲宽度:19μs.此优化条件下,姜酚提取量为14.69 mg/g·DW,比传统溶剂浸提法的9.81 mg/g·DW,提高了49.75%.该研究表明PEF在强化生姜姜酚提取优于其它提取工艺,且具有良好的应用前景.

Abstract

Pulsed electric field(PEF)is a novel non-thermal physical field processing technology used in the sterilization and enzyme inactivation of fluid foods,presenting an substantial amount of research on its applications.In recent years,the superior cell wall-breaking capability of PEF,known as"electroporation,"has attracted increasing attention,and its ability to enhance wall-breaking and intracellular functional component extraction has gradually become a popular research topic.The extraction rates of gingerol from ginger were compared by different extraction processes(traditional solvent extraction technique[TET],ultrasonic-assisted extraction technique[UET],soxhlet extraction technique[SET],and pulsed electric field assisted extraction[PEF]).The parameters for the PEF method of extracting gingerol was then optimized by the response surface method.Results indicated that compared to TET,UET,and SET,the PEF method produced the highest extraction yield of gingerol,reaching 13.36 mg/g·DW.Further steps were taken based on single factor and response surface experiment design,and the optimal process parameters for extracting gingerol from ginger by PEF were obtained as follows:electric field intensity:2.0 kV/cm,pulse frequency:22,and pulse width:19 μs,Under this optimal condition,the extracted amount of gingerol was mg/g·DW,an increase of 49.75%compared to the traditional solvent extraction method(9.81 mg/g·DW).This study shows that PEF is the superior extraction methods in enhancing gingerol extraction from ginger and with great application prospects.

关键词

脉冲电场/生姜/姜酚/提取

Key words

pulsed electric field/ginger/gingerol/extraction

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基金项目

湖南省高新技术产业科技创新引领计划项目(2022GK4014)

湖湘青年英才(2020RC3097)

出版年

2024
现代食品科技
华南理工大学

现代食品科技

CSTPCD北大核心
影响因子:1.07
ISSN:1673-9078
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