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长链菊粉对2,4-二硝基氯苯致小鼠湿疹的改善作用

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探讨长链菊粉对小鼠湿疹的改善作用.采用2,4-二硝基氯苯(DNCB)构建小鼠湿疹模型,给予长链菊粉添加量分别为5%和20%的饲料,持续干预3周后检测小鼠皮肤和结肠组织形态变化、肠道菌群组成、脾脏和胸腺的氧化应激及炎症因子水平.长链菊粉可显著改善湿疹小鼠皮肤角化、棘层增厚等症状,减轻肠道黏膜损伤和炎症反应.长链菊粉干预降低了葡萄球菌属和瘤胃球菌属的相对丰度,增加了双歧杆菌属、乳杆菌属和阿克曼氏菌属的相对丰度.此外,与模型组相比,长链菊粉低、高剂量组胸腺的总超氧化物歧化酶(T-SOD)活性和白细胞介素-10(IL-10)水平显著升高,而丙二醛(MDA)含量及白细胞介素-6(IL-6)水平明显下降.长链菊粉低剂量组脾脏中T-SOD活性显著升高而MDA含量显著降低,长链菊粉高剂量组IL-10水平明显升高而IL-6水平明显下降.综上,长链菊粉能改善DNCB诱导的小鼠湿疹,对湿疹小鼠肠道菌群的组成、免疫器官的氧化损伤及炎症反应有一定的调节作用.
Improving the Effects of Long-chain Inulin on Eczema Induced by 2,4-Dinitrochlorobenzene
Improving the effects of long-chain inulin on eczema in mice was investigated using a model of eczema induced with 2,4-dinitrochlorobenzene and feeding mice a diet containing 5%or 20%long-chain inulin for 3 weeks.The effects of long-chain inulin on eczema were evaluated based on pathological changes in skin and colon tissues,fecal microbiota,oxidative stress status,and inflammation-related factors of the thymus and spleen.The results revealed that the symptoms of skin keratosis and spinous layer thickening were significantly alleviated in both low-and high-dose long-chain inulin intervention groups,along with significant reductions in intestinal mucosal injury and inflammatory responses.The results of 16S rRNA sequencing indicated reductions in the relative abundance of Staphylococcus and Ruminococcus in the low-and high-dose intervention groups,whereas there were increases in the relative abundance of Bifidobacterium,Lactobacillus,and Akkermansia.Furthermore,compared with the model group,low-and high-dose inulin intervention contributed to significant increases in the activity of T-SOD and the levels of IL-10 in the thymus,along with significant reductions in the contents of MDA and levels of IL-6.Similarly,compared with the model group,the spleens of mice in the low-dose inulin intervention group showed significant increases in T-SOD activity and reductions in MDA levels,whereas high-dose inulin intervention promoted significant increases and reductions in IL-10 and IL-6 levels,respectively,in spleens.These results indicate that long-chain inulin has the potential to ameliorate 2,4-dinitrochlorobenzene-induced eczema by regulating the gut microbiota,reducing oxidative stress,and attenuating inflammatory reactions.

long-chain inulineczemagut microbiotaoxidative stressinflammatory factor

李婧文、莫玲、李晶晶、朱银涓

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广西卫健委全生命周期健康与保健研究重点实验室,广西环境暴露组学与全生命周期健康重点实验室,桂林医学院公共卫生学院,广西桂林 541199

江南大学食品学院,江苏无锡 214122

长链菊粉 湿疹 肠道菌群 氧化应激 炎症因子

2024

现代食品科技
华南理工大学

现代食品科技

CSTPCD北大核心
影响因子:1.07
ISSN:1673-9078
年,卷(期):2024.40(11)