摘要
该研究探讨了药食两用八月瓜种子[Akebia trifoliata(Thunb.)Koidz.Seeds]的化学成分及其α-葡萄糖苷酶抑制活性.将八月瓜种子经水和95％乙醇提取,不同极性溶剂萃取后,分别测试各部位的α-葡萄糖苷酶抑制活性,发现95％乙醇提取物的正丁醇部位为α-葡萄糖苷酶抑制活性部位(IC50为18.16 μg/mL),采用硅胶柱色谱、HP20SS柱色谱和半制备HPLC等手段对正丁醇部位进行分离纯化.从八月瓜种子正丁醇部位中分离得到14个化合物,通过与文献波谱数据对比,分别鉴定为:Hederacholichiside F(1)、Saponin E(2)、Mutong Sponin B(3)、Saponin PJ1(4)、Saponin PH(5)、Yuzhizioside Ⅳ(6)、Akebia Saponin D(7)、Saponin B(8)、HN-Saponin F(9)、YuzhiziosideⅣ a(10)、Icariside D(11)、Phenethyl rutinoside(12)、Calceolariolside B(13)和 Isoquercitrin(14).化合物 9、10、11、12、14为首次从八月瓜中分离得到.化合物2、7、10、14对α-葡萄糖苷酶具有一定程度的抑制活性,其IC50分别为17.48、35.62、35.79、44.78 μg/mL.化合物6、13的抑制活性最为显著,其IC50分别为4.27、2.95 μg/mL,活性优于阳性药阿卡波糖(IC50为6.02 μg/mL).
Abstract
In this study,the chemical constituents and α-glucosidase inhibitory activity of medicine and food dual-use Akebia trifoliata(Thunb.)Koidz.Seeds were studied.The seeds of Akebia trifoliata were extracted with water and 95％ethanol,then extracted with different polar solvents.The α-glucosidase inhibitory activity of each of these extract fractions was determined.It was found that the n-butanol fraction of the 95％ethanol extract could inhibit the active site of the α-glucosidase(IC50 was 18.16 μg/mL).The n-butanol fraction was separated and purified by silica gel column chromatography,HP20SS column chromatography and semi-preparative HPLC.Fourteen compounds were isolated from the n-butanol fraction of Akebia trifoliata seeds,and after the comparison with the spectral data in the literature,they were identified as Hederacholichiside F(1),Saponin E(2),Mutong Sponin B(3),Saponin PJ1(4),Saponin PH(5),YuzhiziosisideⅣ(6),Akebia Saponin D(7),Saponin B(8),HN-Saponin F(9),Yuzhizioside Ⅳ a(10),Icariside D(11),Phenethyl rutinoside(12),Calceolariolside B(13)and Isoquercitrin(14).Compounds 9,10,11,12 and 14 were isolated from Akebia trifoliata for the first time.Compounds 2,7,10 and 14 had certain inhibitory activities against α-glucosidase with 1C50 values being 17.48,35.62,35.79 and 44.78 μg/mL,respectively.Compounds 6 and 13 showed the most significant inhibitory activities,with IC50 values being 4.27 μg/mL and 2.95 μg/mL,respectively,which were superior to that of acarbose(IC50:6.02 μg/mL).
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