Chemical Composition and α-Glucosidase Inhibitory Activity of the Seeds of Akebia trifoliata
In this study,the chemical constituents and α-glucosidase inhibitory activity of medicine and food dual-use Akebia trifoliata(Thunb.)Koidz.Seeds were studied.The seeds of Akebia trifoliata were extracted with water and 95％ethanol,then extracted with different polar solvents.The α-glucosidase inhibitory activity of each of these extract fractions was determined.It was found that the n-butanol fraction of the 95％ethanol extract could inhibit the active site of the α-glucosidase(IC50 was 18.16 μg/mL).The n-butanol fraction was separated and purified by silica gel column chromatography,HP20SS column chromatography and semi-preparative HPLC.Fourteen compounds were isolated from the n-butanol fraction of Akebia trifoliata seeds,and after the comparison with the spectral data in the literature,they were identified as Hederacholichiside F(1),Saponin E(2),Mutong Sponin B(3),Saponin PJ1(4),Saponin PH(5),YuzhiziosisideⅣ(6),Akebia Saponin D(7),Saponin B(8),HN-Saponin F(9),Yuzhizioside Ⅳ a(10),Icariside D(11),Phenethyl rutinoside(12),Calceolariolside B(13)and Isoquercitrin(14).Compounds 9,10,11,12 and 14 were isolated from Akebia trifoliata for the first time.Compounds 2,7,10 and 14 had certain inhibitory activities against α-glucosidase with 1C50 values being 17.48,35.62,35.79 and 44.78 μg/mL,respectively.Compounds 6 and 13 showed the most significant inhibitory activities,with IC50 values being 4.27 μg/mL and 2.95 μg/mL,respectively,which were superior to that of acarbose(IC50:6.02 μg/mL).
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