A method for label-free detection of lactic acid in facilitating tumor cell proliferation and migration by high-content screening technology
Objective:To evaluate the effect of high-content screening technology in label-free detection of cell proliferation and migration.Methods:Mouse melanoma B16-F10 cells were cultured in vitro.Different concentrations of lactic acid(2.5,5,10,15,30 mmol/L)were added to the experimental group,respectively,and PBS was added to the control group.High-content screening analysis system was used to take a shot once every 15 minutes for 36 h,and the effects of different concentrations of lactic acid on the proliferation and migration of B16-F10 cells were detected.Results:High-content screening analysis showed that the cell proliferation rate of the experimental group was significantly faster than that of the control group after treatment with different concentrations of lactic acid,and the cell density of the experimental group with 15 and 30 mmol/L lactic acid was apparently higher than that of the other groups.The high-content screening analysis system software was used to count the cells.The results showed that the number of cells in the experimental groups with 15 and 30 mmol/L lactic acid was significantly higher than that in the control group at 12 h after treatment;and with the prolongation of culture time,the number of cells in the experimental groups with 15 and 30 mmol/L lactic acid was significantly higher than that in the control group(all P<0.05).Another finding about the cell migration distance at 0-6 h after treatment demonstrated that,the average migration distance of cells in each experimental group with lactic acid treatment was significantly larger than that in the control group(all P<0.05),while that in the 5 mmol/L lactic acid group was the largest.The proportion of cells with migration behavior in each experimental group was significantly higher than that in the control group.With the increase of lactic acid concentration,the proportion of cells with migration behavior decreased.Conclusion:A rapid,label-free,reliable and accurate method for quantitative evaluation of cell proliferation and migration using high-content screening analysis technology was successfully established.The experimental operation is relatively simple,and intuitive images and diverse statistics can be obtained.
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