基于生物信息学分析MBD1在肝细胞癌中的表达及临床意义
Bioinformatics-based analysis of MBD1 expression and clinical significance in Liver Hepatocellular Carcinoma
彭倩 1陈纪涛 1原倩影 1张依 1刘季芳1
作者信息
- 1. 广州医科大学附属第五医院前沿医学交叉研究中心,广东广州 510700
- 折叠
摘要
目的:通过生信在线数据库分析MBD1在肝细胞癌(LIHC)中的表达及临床意义.方法:通过TIMER2.0、UALCAN、HPA、LinkedOmics数据库分析LIHC和正常肝脏组织中MBD1基因的表达水平及与患者预后的关系,并分析共表达基因和对KEGG信号通路进行富集分析.结果:MBD1在LIHC(371例)中的表达高于正常组织(50例);随着临床分期的升高,MBD1表达呈上升趋势;并且MBD1在TP53突变中表达水平较高(P<0.05).生存分析提示,MBD1高表达的LIHC患者生存率显著降低(P<0.05).共表达分析显示,共有6196个基因与MBD1表达呈显著正相关(P<0.05),正相关性最高的基因为PIAS2,相关系数为0.589;1946个基因与MBD1表达呈显著负相关(P<0.05),负相关性最高的是HEBP2,相关系数是-0.328.KEGG结果表明,MBD1可能与细胞周期、泛素介导的蛋白水解、核糖核酸转运、病毒性致癌等信号通路相关.结论:LIHC组织中MBD1表达增高,MBD1表达升高的LIHC患者预后较差,其可能成为LIHC治疗的新靶点.
Abstract
Objective:To analyze the expression and clinical significance of MBD1 in Liver Hepatocellular Carcinoma(LIHC)based on bioinformatics online databases.Methods:TIMER2.0,UALCAN,HPA and LinkedOmics databases were used to analyze the expression level of MBD1 gene in LIHC and normal liver tissues and its relationship with patient prognosis,co-expressed genes were analysed and enrichment analysis of KEGG signaling pathways was also performed.Results:The expression of MBD1 in LIHC tissues(371 cases)was higher than that in normal tissues(50 cases);the expression of MBD1 increased with the increase of clinical stage;moreover,the expression level of MBD1 was higher in TP53 mutations(P<0.05).Survival analysis showed that the survival probability of LIHC patients with high expression of MBD1 were significantly poor(P<0.05).Co-expression analysis showed that a total of 6196 genes were significantly and positively correlated with MBD1 expression(P<0.05),and the gene with the highest positive correlation expression was PIAS2,with a correlation coefficient of 0.589;1946 genes were significantly and negatively correlated with MBD1 expression(P<0.05),with HEBP2 having the highest negative correlation expression,with a correlation coefficient of-0.328.KEGG results showed that MBD1 may be related to cell cycle,ubiquitin mediated proteolysis,RNA transport,viral carcinogenesis,and other signaling pathways.Conclusion:The expression level of MBD1 in LIHC tissues is increased,and the prognosis of LIHC patients with high expression of MBD1 is poor,which may become a new therapeutic target for LIHC.
关键词
临床预后/肝细胞癌/MBD1/富集分析Key words
clinical prognosis/liver hepatocellular carcinoma/MBD1/enrichment analysis引用本文复制引用
基金项目
国家自然科学基金面上项目(81772553)
广州市西医类一般引导项目(20211A011104)
出版年
2024
NETL
NSTL
万方数据