Decursin inhibits apoptosis of nucleus pulposus cells by activating the PPAR signaling pathway
Objective:To investigate the effect and mechanism of decursin(DE)on inhibiting apoptosis of nucleus pulposus cells.Methods:Primary rat nucleus pulposus cells were cultured in vitro,and tumor necrosis factor-α(TNF-α)was used to construct an intervertebral disc degeneration(IDD)model.CCK-8 assay was used to detect the effect of different concentrations of DE on the viability of nucleus pulposus cells in IDD model,and the optimal experimental concentration of DE was determined for subsequent experiments.The effects of DE on apoptosis of nucleus pulposus cells were detected by TUNEL staining,flow cytometry and Western blotting.The difference of gene expression between the model group and the DE treatment group was detected by transcriptome sequencing(RNA-seq),and analyze by Limma package.Additionally,the changes in signaling pathways between the two groups were examined through GSEA analysis and further validated experimentally.Results:DE could increase the viability of nucleus pulposus cells and inhibit the apoptosis of nucleus pulposus cells in IDD model(all P<0.05).RNA-seq results suggested that DE could activate the peroxisome proliferator-activated receptor(PPAR)signaling pathway.Further studies confirmed that DE increased the viability of nucleus pulposus cells and reduced the apoptosis of nucleus pulposus cells by up-regulating PPARα expression(all P<0.05).Conclusion:DE inhibits apoptosis of nucleus pulposus cells in IDD model by activating PPAR signaling pathway.
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