共刺激分子在复感儿中医分型中的表达研究
Clinical Significance of Costimulatory Molecule Expression in Syndrome Differentiation of Recurrent Respiratory Tract Infection Children
章文平 1廖永州 1何胜晓 1李宜瑞1
作者信息
- 1. 广州中医药大学第一附属医院儿科,广东广州510405
- 折叠
摘要
[目的]通过观察反复呼吸道感染(RRTI)非急性感染期肺脾气虚、肺脾气阴两虚型患儿(简称"复感儿")T细胞CD8+CD28+的表达,探讨两个证型T淋巴细胞亚群的表达特点及临床意义,为RRll的中医辨证提供可能的物质基础及客观依据.[方法]选择RRTI非急性期肺脾气虚、肺脾气阴两虚患儿各30例,运用流式细胞仪对RRTI患儿外周血T淋巴细胞亚群表达进行检测,并与正常对照组30例比较,结果运用sPss/PC 10.O统计软件包进行分析处理.[结果]与正常对照组比较,RRTI的CD8+、CD28+、CD8+CD28+%、CD8+CD28+表达降低,而CD8+CD28-%、CD8+ CD28-表达增高.RRTI不同中医分型T细胞亚群的表达也不同.经多元回归分析,中医分型证候积分与CD8+CD28-%、CD8+CD28-%的表达相关.[结论]T淋巴细胞亚群失衡为RRTI发病机制之一,且与RRTl非急性感染期肺脾气虚证与肺脾气阴两虚证的证候积分相关,这可能将有助于中医辨证分型.
Abstract
Objective To observe T lymphocytes CD+8 CD+28 expression in recurrent respiratory tract infection (RRTI) children with the syndromes of lung-spleen Qi deficiency and lung-spleen Qi-yin deficiency during the non-acute infective stage, and to investigate the relationship of T lymphocytes subtypes distribution with syndrome differentiation of RRTI. Methods Peripheral T lymphocyte CD+8 CD+28 expression was detected by flow cytometry in 30 RRTI children with lung-spleen Qi deficiency and in 30 RRTI children with lung-spleen Qi-yin deficiency. The results were compared with those in 30 healthy children, and then were analyzed with SPSS/PC10. 0 software.Results Compared with the healthy children, the percentages of CD+8, CD+28 and CD+8 CD+28 as well as CD+8 CD+28 expression were decreased, while CD+8 CD-28 percentage and CD+8 CD-8 expression were increased. The distribution of T lymphocytes subtypes varied in RRTI with different syndrome patterns. The results of multiple regression analysis showed that the scores of syndromes were correlated with the percentages of CD+8 CD+28 and CD+8 CD-28. Conclusion The imbalance of the distribution of T lymphocyte subtypes is one of the pathogenesis of RRTI. The expression of T lymphocyte subtypes is correlated with the syndrome scores of RRTI children with the syndromes of lung-spleen Qi deficiency and lung-spleen Qi-yin deficiency during the non-acute infective stage,which will be helpful for the syndrome differentiation of RRTI in children.
关键词
反复呼吸道感染/中医病机/肺脾气虚/肺脾气阴两虚/T淋巴细胞亚群/儿童Key words
RECURRENT RESPIRATORY TRACT INFECTION/pathogenesis (TCM)/LUNG-SPLEEN QI DEFICIENCY/LUNG-SPLEEN QI-YIN DEFICIENCY/T LYMPHOCYTE SUBTYPES/CHILDREN引用本文复制引用
基金项目
广东省中医药局科技项目(20070432)
出版年
2011
国家科技期刊平台
NSTL
维普
万方数据