Objective To screen transgenic Houttuynia cordata Thunb. and to analyze the stability of exogenous genes initially. Methods Kanamycin was smeared to the surface of the ]eaves to screen the transgenic plants containing the npt Ⅱ gene. And then PCR detection of the npt Ⅱ gene and target gene CN (cecropin B/NP-1 )was carried out in kanamycin-resistance plant. Results The results of kanamycin-smearing screening method showed that the optimal kanamycin concentration was 5000 mg/L and the optimal observation time was day 1 to day 4. The result of PCR analysis showed that the expression ratio of resistant gene npt Ⅱ was 96％ in the resistant plants, and the expression ratio of target gene CN was 37％. Conclusion A method for quickly screening transgenic Houttuynia cordata Thunb. has been established. The stability of target gene in the transgenic plant is inferior to the selecting maker gene.
NETL
NSTL
万方数据
维普
