肾阴虚证血浆蛋白质谱的差异表达
Differential Expression of Plasma Protein Profile in Patients with Kidney-yin Deficiency Syndrome
毕建璐 1魏敏 2孙晓敏 3聂晓莉 3肖雅 4莫伟 1赵晓山 3罗仁3
作者信息
- 1. 广东省第二中医院,广东广州510095
- 2. 福建中医药大学,福建福州350122
- 3. 南方医科大学中医药学院,广东广州510515
- 4. 暨南大学医学院,广东广州510632
- 折叠
摘要
[目的]寻找肾阴虚证差异表达的血浆蛋白质,并筛选其特异性表达蛋白,为建立中医肾虚证证候客观化标准提供指标. [方法]选取典型肾阴虚证患者5例、正常健康志愿者6例,对2组血浆进行抗体芯片表达谱比较研究. [结果]正常对照组与肾阴虚证组间的差异表达蛋白有25条.其中,表达上调的蛋白有2条,表达下调的蛋白有23条. [结论]肾阴虚证差异表达的蛋白主要涉及到免疫紊乱、蛋白质生物合成、新陈代谢、氧化应激、细胞凋亡、细胞信号传导等相关方面.
Abstract
Objective To seek for the differentially-expressed proteins in patients with kidney-yin deficiency syndrome and to screen out the specific proteins,so as to provide evidence for the establishment of objective standard of kidney deficiency syndrome of traditional Chinese medicine (TCM).Methods Five patients with typical kidney-yin deficiency syndrome and 6 normal healthy volunteers were enrolled into the study.Plasma proteins in both groups were detected by antibody chip,and then the plasma proteins profile was compared and analyzed.Results A total of 25 differentially-expressed proteins between kidney-yin deficiency group and normal control group were found,of which 2 were up-regulated and 23 were down-regulated.Conclusion The differentially-expressed proteins in patients with kidney-yin deficiency syndrome are mainly related to immune disorder,protein biosynthesis,metabolism,oxidative stress,cell apoptosis,signal transduction,and so on.
关键词
肾阴虚证/中医证候/蛋白质组学/芯片Key words
kidney-yin deficiency syndrome/TCM syndromes/proteomics/chips引用本文复制引用
基金项目
国家自然科学基金(81373707)
国家自然科学基金(81603520)
国家自然科学基金(81673960)
国家自然科学基金(81573812)
广东省自然科学基金(2014A030313292)
广东省自然科学基金(2015A030313300)
广东省自然科学基金(2016A0303130084)
广东省优秀青年教师培养计划项目(YQ2015035)
广东省省级科技计划项目(2014A020221073)
广州市科技计划项目(201607010224)
广州市产学研重大专项(2014Y2-00504)
出版年
2017
国家科技期刊平台
NSTL
维普
万方数据