黄芪甲苷对高糖诱导人绒毛膜滋养层细胞(HTR-8/SVneo)焦亡及侵袭迁移的影响
Effects of Astragaloside Ⅳ on High Glucose-Induced Pyroptosis and Invasive Migration of Human Chorionic Trophoblast Cells(HTR-8/SVneo)
叶文慧 1肖海侠 2蔡双明3
作者信息
- 1. 广东省妇幼保健院内科,广东广州 511442
- 2. 广东省妇幼保健院产科,广东广州 511442
- 3. 广东省妇幼保健院MICU,广东广州 511442
- 折叠
摘要
[目的]探讨黄芪甲苷对高糖诱导的人绒毛膜滋养层细胞(HTR-8/SVneo)焦亡及侵袭迁移的影响.[方法]将HTR-8/SVneo细胞分为4组:对照组(未处理)、高糖组(高糖刺激)及黄芪甲苷 50、100 μmol/L组(高糖刺激+黄芪甲苷).采用细胞计数试剂盒8(CCK-8)法检测细胞活力,分别采用Transwell实验和划痕实验测定细胞侵袭、迁移能力,Hoechst 33342/碘化丙啶(PI)双荧光染色评估细胞焦亡情况,蛋白免疫印迹(Western Blot)法检测细胞NOD样受体热蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶剪切体(cleaved-Caspase-1)、消皮素D-N端(GSDMD-NT)、白细胞介素18(IL-18)蛋白表达水平.[结果]与对照组比较,高糖组HTR-8/SVneo细胞活力显著降低,细胞迁移率显著降低,侵袭细胞数显著减少,PI阳性细胞比例显著增加,NLRP3、cleaved-Caspase-1、GSDMD-NT、IL-18蛋白表达水平显著升高(P＜0.05或P＜0.01);与高糖组比较,黄芪甲苷处理组细胞活力显著升高,细胞迁移率显著增加,侵袭细胞数显著增加,PI阳性细胞比例显著下降,NLRP3、cleaved-Caspase-1、GSDMD-NT、IL-18蛋白表达水平显著下降(P＜0.05或P＜0.01).[结论]黄芪甲苷可抑制高糖诱导的HTR-8/SVneo细胞焦亡,改善细胞侵袭及迁移能力.
Abstract
Objective To investigate the effect of astragaloside Ⅳ on high glucose-induced pyroptosis and invasive migration of human chorionic trophoblast cells(HTR-8/SVneo).Methods HTR-8/SVneo cells were divided into 4 groups:control group(untreated),high glucose group(high glucose stimulation)and astragaloside Ⅳ 50 and 100 μmol/L group(high glucose stimulation + astragaloside).Cell activity was detected by Cell Counting Kit 8(CCK-8),cell invasion and migration abilities were determined by Transwell assay and scratch assay,respectively,cell pyroptosis was assessed by Hoechst 33342/propidium iodide(PI)dual fluorescence staining.The protein expression levels of NOD-like receptor thermoprotein structural domain(NLRP3),cleaved-Caspase-1,GSDMD-NT,and IL-18 were detected by Western Blot.Results Compared with the control group,HTR-8/SVneo cell viability was significantly reduced in the high glucose group,the rate of cell migration was significantly reduced,the number of invasive cells was significantly reduced,the percentage of PI-positive cells was significantly increased,and the levels of NLRP3,cleaved-Caspase-1,GSDMD-NT and IL-18 protein expression levels were significantly increased(P<0.05 or P<0.01);compared with the high glucose group,cell viability was significantly higher in the astragaloside Ⅳ treated group,the rate of cell migration was significantly increased,the number of invasive cells was significantly increased,the percentage of PI-positive cells was significantly decreased,and the protein expressions of number of NLRP3,cleaved-Caspase-1,GSDMD-NT,IL-18 were significantly decreased(P<0.05 or P<0.01).Conclusion AstragalosideⅣcan inhibit high glucose-induced HTR-8/SVneo cell pyrolysis and improve cell invasion and migration ability.
关键词
黄芪甲苷/妊娠期糖尿病/细胞焦亡/细胞侵袭/细胞迁移/人绒毛膜滋养层细胞Key words
astragalosideⅣ/gestational diabetes mellitus/pyroptosis/invasion/migration/human chorionic trophoblast cells引用本文复制引用
基金项目
广东省中医药局科研项目(20221047)
第二批全国西医学习中医优秀人才研究项目(国中医药任教函[2023]90号)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据