芪丹颗粒通过促进CTRP3激活PI3K/AKT信号通路改善脂肪细胞胰岛素抵抗

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中文摘要：[目的]探究芪丹颗粒抗脂肪细胞胰岛素抵抗(IR)机制.[方法]采用棕榈酸培养法诱导3T3-L1脂肪细胞IR模型,设空白对照组,模型组,芪丹颗粒低、中、高剂量组,芪丹颗粒+C1q/TNF相关蛋白3(CTRP3)siRNA组.测定细胞上清中葡萄糖消耗量;采用酶联免疫吸附分析(ELISA)法测定细胞上清中肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)含量;实时定量聚合酶链反应(qRT-PCR)法检测细胞CTRP3、TNF-α、IL-6、葡萄糖转运蛋白4(GLUT4)基因表达;Western Blot法检测细胞中CTRP3、磷脂酰肌醇-3激酶(PI3K)、蛋白激酶B(AKT)的蛋白表达及其磷酸化水平.[结果]与模型组比较,芪丹颗粒中、高剂量组的葡萄糖消耗量升高并趋于空白对照组的70％～82％,TNF-α含量降低了 25％～45％,IL-6含量下降了 40％～55％,GLUT4mRNA表达水平增加了 50％,CTRP3 mRNA及蛋白表达水平增加了 25％～40％(均P＜0.05);与芪丹颗粒组比较,芪丹颗粒+CTRP3 siRNA组的葡萄糖消耗量和GLUT4 mRNA表达水平分别降低了 33％和27％,IL-6和TNF-α的含量分别增加了42％和41％,p-PI3K和p-AKT的蛋白表达水平增加了 190％和180％(均P＜0.05).[结论]芪丹颗粒可能通过促进CTRP3表达降低炎症反应和激活PI3K/AKT信号通路来提高IR 3T3-L1脂肪细胞的胰岛素敏感性,改善脂肪细胞IR.

外文标题：Qidan Granules Improves Insulin Resistance of Adipocytes by Promoting CTRP3 to Activate PI3K/AKT Signaling Pathway

外文摘要：Objective To explore the mechanism of Qidan Granules prevent insulin resistance(IR)in adipocytes.Methods The IR model of 3T3-L1 adipocytes was induced by palmitic acid culture method.The blank control group,model group,Qidan Granules low-,medium-and high-dose groups,Qidan Granules+C1q/TNF-related protein 3(CTRP3)siRNA group were set up.Determination of glucose consumption in cell supernatant was performed;the levels of tumor necrosis factor α(TNF-α)and interleukin 6(IL-6)in the cell supernatant were determined by enzyme-linked immunosorbent assay(ELISA).Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the gene expressions of CTRP3,TNF-α,IL-6 and glucose transporter 4(GLUT4).The protein expressions and phosphorylation levels of CTRP3,phosphoinositide-3 kinase(PI3K)and protein kinase B(AKT)were detected by Western Blot.Results Compared with the model group,the glucose consumption was increased and tended to 70％-82％of the blank control group,TNF-α content was decreased by 25％-45％,IL-6 content was decreased by 40％-55％,GLUT4 mRNA expression level was increased by 50％,and mRNA and protein expression levels of CTRP3 were increased by 25％-40％in the medium-and high-dose groups of Qidan Granules(all P＜0.05).Compared with the Qidan Granules group,the glucose consumption and GLUT4 mRNA expression level in the Qidan Granules+CTRP3 siRNA group were decreased by 33％and 27％,respectively,the contents of IL-6 and TNF-α were increased by 42％and 41％,respectively,and the protein expressions of p-PI3K and p-AKT were increased by 190％and 180％,respectively(all P＜0.05).Conclusion Qidan Granules may improve the insulin sensitivity of IR 3T3-L1 adipocytes and improve IR of adipocytes by promoting CTRP3 expression to reduce inflammatory response and activate PI3K/AKT signaling pathway.

外文关键词：

Qidan granulestype 2 diabetes mellitus(T2DM)insulin resistanceC1q/TNF-related protein 3(CTRP3)PI3K/AKT signaling pathwayinflammation3T3-L1 adipocytes

作者：

张越、郭叙喜、叶仁群

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作者单位：

深圳市宝安区中医院内分泌科,广东深圳 518100

关键词：

芪丹颗粒 2型糖尿病胰岛素抵抗 C1q/TNF相关蛋白3(CTRP3) PI3K/AKT信号通路炎症 3T3-L1脂肪细胞

基金：

深圳市科技计划深圳市宝安区科技计划

项目编号：

JCYJ201908071108036242021JD276

出版年：

2024

DOI：

10.13359/j.cnki.gzxbtcm.2024.06.031

广州中医药大学学报

广州中医药大学

广州中医药大学学报

CSTPCD

影响因子：1.055

ISSN：1007-3213

年,卷(期)：2024.41(6)