广陈皮高效液相色谱陈化特征图谱研究
Study on HPLC Aging Characteristic Chromatogram of Guangdong Citri Reticulatae Pericarpium
陈明权 1商雪莹 1李华 2覃仁安 1何风雷1
作者信息
- 1. 广州白云山陈李济药厂有限公司,广东广州 510288
- 2. 广东省药品检验所,广东广州 510663
- 折叠
摘要
[目的]建立广陈皮的高效液相色谱(HPLC)陈化特征图谱.[方法]采用HPLC-二极管阵列检测器(DAD)技术分析不同年份广陈皮之间的成分差异,测定各差异色谱峰的相对峰面积,通过聚类分析对其进行陈化等级分类,并通过中药色谱指纹图谱相似度评价系统(2012A 版)进行评价.[结果]不同年份广陈皮被聚为Ⅰ、Ⅱ类两个不同的陈化等级,Ⅰ类(低陈化等级)广陈皮的相似度为0.708～0.957,Ⅱ类(高陈化等级)广陈皮的相似度为0.734～0.976,且两者之间的相似度均低于0.6.建立的特征图谱中存在9个差异色谱峰;Ⅱ类广陈皮的对照特征图谱T2可视为其陈化特征图谱,其中1～4号峰、9号峰为陈化特征峰.[结论]建立的广陈皮陈化特征图谱操作简单,具有良好的重复性与可靠性,可用于不同年份广陈皮的质量评价.
Abstract
Objective To establish a high performance liquid chromatography(HPLC)aging characteristic chromatogram of Guangdong Citri Reticulatae Pericarpium.Methods HPLC-diode array detector(DAD)was used to analyze the composition differences between different years of Guangdong Citri Reticulatae Pericarpium,and the relative peak area of each differential chromatographic peak was determined.The aging grade was classified by cluster analysis,and evaluated by the similarity evaluation system for chromatographic fingerprint of TCM(2012A edition).Results The different years of Guangdong Citri Reticulatae Pericarpium was clustered into two different aging grades of classⅠ and class Ⅱ.The similarity of class Ⅰ(low aging grade)Guangdong Citri Reticulatae Pericarpium was 0.708-0.957,and the similarity of class Ⅱ(high aging grade)Guangdong Citri Reticulatae Pericarpium was 0.734-0.976,and the similarity between the two was lower than 0.6.There were 9 different chromatographic peaks in the established characteristic chromatogram.The control characteristic spectrum T2 of typeⅡ Guangdong Citri Reticulatae Pericarpium can be regarded as its aging characteristic spectrum,among which peaks 1-4 and 9 are aging characteristic peaks.Conclusion The established aging characteristic chromatogram of Guangdong Citri Reticulatae Pericarpium is simple in operation,with good repeatability and reliability,and can be used for quality evaluation of different years of Guangdong Citri Reticulatae Pericarpium.
关键词
广陈皮/陈化/高效液相色谱-二极管阵列检测器(HPLC-DAD)/特征图谱/维采宁-2/聚类分析/质量控制Key words
Guangdong Citri Reticulatae Pericarpium/aging/high performance liquid chromatography-diode array detector(HPLC-DAD)/characteristic spectrum/Vicerenin-2/cluster analysis/quality control引用本文复制引用
基金项目
国家药品监督管理局药品快速检验技术重点实验室开放性课题(KF2021005)
广州市科技计划项目(201710010122)
出版年
2024
国家科技期刊平台
NSTL
万方数据